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Effect of taurine on GFAP and TauT expressions in rat retinal Müller cells in high glucose culture 被引量:1

Effect of taurine on GFAP and TauT expressions in rat retinal Müller cells in high glucose culture
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摘要 Objective: To detect the expression of glial fibrillary acid protein (GFAP) and taurine transporter (TauT) in the retinal Müller cells in high glucose culture with taurine and to explore the influence of glucose on the taurine transporting, and the possible protective effects of taurine on MUller cells in early diabetic retinopathy. Methods: The Müller cells from the rat retina were cultured in high glucose, and GFAP and Taut expressions were detected in the cells treated with different doses of taurine by immuocytochemical fluorescein staining and Western blotting. Results: High glucose enhanced the expression of GFAP and decreased the expression of TauT in Müller cells. Taurine decreased the up-regulation of GFAP in the cells which was induced by high glucose; 0. 1-10 mmol/L taurine increased the expression of TauT in Müller cells. Conclusion: Taurine can inhibit the changes in Müller cell resulted from high glucose. Objective:To detect the expression of glial fibrillary acid protein (GFAP) and taurine trans- porter (TauT) in the retinal Müller cells in high glucose culture with taurine and to explore the influence of glucose on the taurine transporting,and the possible protective effects of taurine on Müller cells in early diabetic retinopathy.Methods.The Müller cells from the rat retina were cultured in high glucose,and GFAP and TauT expressions were detected in the cells treated with different doses of taurine by immuocy- tochemical fluorescein staining and Western blotting.Results:High glucose enhanced the expression of GFAP and decreased the expression of TauT in Müller cells.Taurine decreased the up-regulation of GFAP in the cells which was induced by high glucoses 0.1-10mmol/L taurine increased the expression of TauT in Müller cells.Conclusion:Taurine can inhibit the changes in Müller cell resulted from high glucose.
出处 《Journal of Medical Colleges of PLA(China)》 CAS 2007年第3期137-142,共6页 中国人民解放军军医大学学报(英文版)
基金 Supported by the National Natural Science Foundation of China(No. 30571570)
关键词 Müller cells TAURINE taurine transporter glial fibrillary acid protein immunocytochemical fluorescein staining 小鼠 动物实验 神经胶质 免疫荧光反应
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