摘要
目的:观察谷氨酸钠不同给药时程对幼年豚鼠视网膜神经节细胞损伤的程度,以建立简便而稳定可靠的视网膜兴奋毒性损伤模型。方法:实验于2005-03/2006-06在泰山医学院动物实验中心和形态学实验室完成。①实验材料:45～50d(幼年)豚鼠35只,体质量280～320g,雌雄不拘;谷氨酸钠(上海伯奥生物科技有限公司生产),纯度99%,pH6.9～7.2,用生理盐水配成300g/L溶液。②实验分组:采用随机数字法将豚鼠分成2组,即正常对照组5只、谷氨酸钠组30只(谷氨酸钠组根据用药时间又分为4个亚组,即1d组5只、3d组6只、7d组9只,14d组10只)。③实验干预:谷氨酸钠组按3g/kg体质量腹腔注射谷氨酸钠,分别连续1,3,7和14d。④实验评估:给药后,再饲养10d后取材,观察视网膜神经节细胞数目和形态学的变化。结果:①各组豚鼠视网膜光镜观察结果:谷氨酸钠1d组视网膜神经节细胞未见明显形态学改变;3d组少数细胞发生皱缩;7d组视网膜神经节细胞损伤较为明显,多数神经节细胞发生核固缩;14d组动物半数死亡(5/10),视网膜神经节细胞损伤严重,明显脱失,残留细胞体积变小。②各组豚鼠视网膜神经节细胞密度:谷氨酸钠14d组、7d组视网膜神经节细胞密度与正常对照组比较差异均显著(P<0.01),谷氨酸钠3d组与正常对照组比较差别有统计学意义(P<0.05),谷氨酸钠1d组与正常对照组比较差异无显著性(P>0.05)。结论:谷氨酸钠对幼年豚鼠视网膜的毒性损伤与用药时程有关,按3g/kg体质量腹腔注射,1次/d,连续7d,能成功建立谷氨酸视网膜兴奋毒性豚鼠动物模型,且无一例死亡,损伤适中。
AIM： To observe the effect of sodium glutamate （MSG） by different administration time on the injury degree of retinal ganglial calls in juvenile guinea pig, and establish an ideal retinal excitotoxicity model. METHODS： The experiment was performed in the Animal Experimental Center and Morphological Laboratory of Taishan Medical College from March 2005 to June 2006. （1）Thirty-five 45 to 50-day-old juvenile guinea pigs （280-320 g, either male or female） were randomly divided into 2 groups： normal control group （n =5）, and MSG group （n =30, Shanghai Bio Life Science and Technology Co., Ltd. purity of 99%, pH 6.9-7.2, 300 g/L solution mixed with normal saline）. MSG group was subdivided into MSG1 （n =5）, 3 （n =6）, 7 （n =9）, and 14 （n =10） groups according to administration time. The guinea pigs of MSG groups were intraperitoneaUy injected with glutamate （3 g/kg body mass） for 1, 3, 7, and 14 days. （2）All animals were killed and sampled 10 days after injection. The number of retinal ganglion cells （RGCs） and changes in morphology were measured. RESULTS： （1）By optical microscopic study, no obvious change in morphology was found in MSG 1 day group; in MSG 3 day group, some calls shrank; obvious damage of RGCs was observed in MSG 7 day group, and karyopycnosis appeared in majority of RGCs; in MSG 14 day group, the damage of RGCs was severer, the number and size of RGCs were remarkably reduced, and the mortality rate was 5/10. （2）There were significant differences in density rates of RGCs between MSG 7 and 14 groups and normal control group （P〈 0.01）; the density rate in MSG 3 day group was different from that of normal control group （P 〈 0.05）. No statistical difference was found between MSG1 day group and normal control group （P 〉 0.05）. CONCLUSION： Damage of RGCs in juvenile guinea pigs is related to the duration of sodium glutamate, and the intraperitoneal injection of 3 g/kg body mass, once daily for 7 days is an ideal administration time to establish a glutamate dependent retinal excitotoxicity model with proper damage and no death.
出处
《中国组织工程研究与临床康复》
CAS
CSCD
北大核心
2007年第27期5432-5434,共3页
Journal of Clinical Rehabilitative Tissue Engineering Research
