摘要
目的：建立肝再生刺激物受体（ＨＳＳＲ）的荧光配体分析法，探讨该反应系统的最适反应条件，分析ＨＳＳ与ＨＳＳＲ结合的特异性。方法：用己硫氰酸荧光素（ＦＩＴＣ）标记ＨＳＳ，通过直接荧光法观察。结果：肝细胞浓度为５×１０６／ｍｌ，ＦＩＴＣＨＳＳ１∶４稀释，４℃反应４ｈ即可得到满意结果。结论：肝细胞膜上存在ＨＳＳＲ，对进一步探讨ＨＳＳ作用的分子生物学机理具有重要意义。
Objective:To detect the hepatocyte stimulating substance receptors (HSSR) on fetus hepatocytes primary culture with fluoresceinligand binding assay.Method:HSS labelled with fluorescein isothiocyanate (FITC) was used for staining the hepatocytes.Results:Satisfactory results were observed with hepatocytes in concentration of 5×10 5/ml,FITC HSS in 1∶4 dilution and kept at 4℃ for 4 h.The HSSR was shown to be located on membrances of hepatocytes.Conclusion:This assay is important for studying the mechanism of HSS on hepatocytes.
出处
《蚌埠医学院学报》
CAS
1997年第2期127-128,共2页
Journal of Bengbu Medical College
关键词
肝再生刺激物
药物疗法
荧光配体结合法
liver regeneration
receptor
hepatocyte stimulating substance
hepatocyte
