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氧化砷对白血病细胞内 PML/PML-RARα 蛋白的影响 被引量:33

Effects of arsenic trioxide on the subcellular localization of PML/PML RAR α protein in leukemic cells
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摘要 目的:探讨急性早幼粒细胞白血病细胞的分子标志PML-RARα在氧化砷(As2O3)诱导的细胞凋亡中的可能作用。方法:观察As2O3对PML-RARα蛋白在亚细胞定位的影响。结果:①在1μmol/L的As2O3作用下,HL-60细胞核内PML抗血清染色明显减少,而在胞浆的近核膜区出现弥散分布的荧光染色;②As2O3诱导NB4细胞内散在分布的PML抗血清染色颗粒明显减少;③通常情况下,少数NB4细胞的胞浆中存在PML抗血清染色颗粒的聚集,这类细胞在As2O3作用后明显增多,凋亡细胞中PML抗血清染色聚集现象也存在;④全反式维甲酸(ATRA)预处理24或48小时并不改变As2O3对NB4细胞的凋亡诱导效应。结论:As2O3诱导细胞凋亡的过程不依赖维甲酸信号途径,可能通过PML/PML-RARα及其它相关基因或蛋白的调控来实现。 Objective:In order to illustrate the possible roles of PML RAR α protein in arsenic trioxide(As 2O 3) induced NB4 cell apoptosis.Methods: Effects of As 2O 3 on the subcellular localization of PML RARα in NB4 cells were studied.Results:① Anti PML serum staining was reduced and PML granules emerged in the perinuclear cytoplasm in a diffuse pattern in HL 60 cells under As 2O 3 treatment; ② abnormal PML/PML RAR α granules were decreased;③ NB4 cells accumulated anti PML serum staining granules in the cytoplasms were increased and similar accumulation also found in apoptotic cells;and ④ pretreatment with all trans retinoic acid (ATRA) for 24 or 48 hours did not alter the As 2O 3 effects.Conclusion: As 2O 3 induced apoptosis was independent of the retinoic acid signal pathway,and it might be regulated by PML/PML RAR α and/or other related genes.
出处 《中华血液学杂志》 CAS CSCD 北大核心 1997年第1期32-34,共3页 Chinese Journal of Hematology
基金 国家自然科学基金 卫生部优秀青年人才基金 上海市科委重点项目 启明星计划 上海血液学研究所胡应洲基金
关键词 氧化砷 蛋白 急性白血病 Arsenic trioxide PML RARα protein Leukemia,myelocytic,acute
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参考文献4

  • 1陈国强,中华血液学杂志,1997年,18卷,25页
  • 2Chang K S,Blood,1995年,85卷,3646页
  • 3朱军,Leukemia,1995年,9卷,302页
  • 4陈国强,中华血液学杂志,1995年,16卷,44页

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