摘要
目的探讨二噁英（TCDD）对大鼠成骨细胞增殖及分泌功能的调控作用。方法应用1×10^-10～1×10^-8mol/L浓度的二噁英作用原代培养的大鼠颅盖骨成骨细胞24 h;采用四甲基偶氮噻唑蓝（MTT）法检测二噁英对成骨细胞增殖能力的影响;采用对硝基酚磷酸盐法测定成骨细胞培养液碱性磷酸酶（ALP）浓度。结果二噁英对大鼠成骨细胞增殖和分泌功能有负性调节作用,与对照组比较,1×10^-8mol/L的二噁英使大鼠成骨细胞增殖能力降低60%,使成骨细胞ALP活性减少58%,差异有统计学意义（P〈0.05）。结论二噁英对大鼠颅盖骨成骨细胞中成骨细胞增殖和分泌功能具有负性调控作用。
Objective To investigate the effect of 2, 3, 7, 8-tetrachorodibenzo-p-dioxin（TCDD） on the cell proliferation and secretion in rat osteoblasts. Methods The osteoblasts from rat cranium were cultured with TCDD（1 ×10^-10～1 x 10 -8 mol/L） for 24 hours.The nitrophenol phcsphate salt method was used to measure the alkaline phosphatase（ALP） content within osteoblasts; The MTT assay was used to assess the cell proliferation. Results The cell proliferation and secretion in rat osteoblasts were down-regulated by TCDD. The cell number in rat osteoblasts exposed to TCDD at 1 ×10^-6 mol/L increased 60% compared with control group（P 〈0.05）.ALP activity in at 1 ×10^-6mol/L ostcoblasts was higher 58% than that without TCDD-treated（P〈0.05）.Conclusion The down-regulation may be correlated with TCDD modulation to the cell proliferation and secretion in rat osteoblasts.
出处
《中国公共卫生》
CAS
CSCD
北大核心
2007年第7期876-877,共2页
Chinese Journal of Public Health
基金
国家自然科学基金(30500414)
辽宁省教育厅高等学校科学研究项目(05L508)
关键词
二噁英
成骨细胞
细胞增殖
dioxin
osteoblasts
cell proliforation
