摘要
本文研究建立了人血清中氯林可霉素的高效液相色谱测定法（ＨＰＬＣ），该方法在超低紫外（１９８ｎｍ）下测定，具有取样量小（２００μｇ），线性范围宽（０．５％～５０μｇ／ｍｌ）；灵敏度高，以３倍基线噪音计算，最低检测限可达０．０５μｇ／ｍｌ；重现性好，高中低３个质控样品批间ＲＳＤ％分别为１．９７、０．４０和２．１５，批内ＲＳＤ％分别为０．８０、０．４６和２．３３，平均回收率分别为１０１．８７，１００．３４％和９３．８１％；方法操作简便等特点，为测定血清中药物浓度，确定给药方案和治疗剂量。
This paper reported a simple,rapid and sensitive HPLC method for the determination of clindamycin in human serum(0.2ml).The method involved a protein precipitation with acetonitrile and quantification by RP HPLC with ultra UV (198nm) detection.The assay allowed excellent linearity in the range of 0.5-50mg/L,and the limit of sensitivity for the assay was 0.05mg/L.The coefficient of variation of three check samples(2,20 and 35mg/L) analyzed on a intra day (n=10) were 2.33%,0.46% and 0.80%;the inter day (n=6) were 2.15%,0.40% and 1.97% ,respectively;and the mean recovery were 93.81%,100.34% and 101.87%,respectively.This method has successfully been applied to the analysis of serum samples taken up to 8 or 12 hours after intravenous bolus infusions and oral administration of 300￣600mg in healthy volunteers.
出处
《中国医院药学杂志》
CAS
CSCD
北大核心
1997年第2期69-71,共3页
Chinese Journal of Hospital Pharmacy
