摘要
本文比较了营养琼脂培养基、改良的GM培养基以及十分之一改良GM培养基在10%粗盐的选择压力下对嗜盐及耐盐菌的分离效果,用筛选出的最佳培养基对塔里木盆地40多种土样的嗜盐及耐盐菌进行了分离。结果表明:塔里木盆地嗜盐及耐盐菌主要是一些贫营养类群,十分之一改良的GM培养基分离效果最好。塔里木盆地的嗜盐及耐盐菌其数量在103～105cfu/g之间,且表现为农田>荒漠>沼泽沉积物,植物根际>林地,而不同菌落的种类表现为农田>沼泽沉积物>林地,植物根际>荒漠。分离得到的嗜盐及耐盐菌数量与土样中养分及盐分离子的含量之间并没有显著的相关关系表明不同生境下的嗜盐及耐盐菌可能具有不同种类。
In this study, the media of nutrient agar, modified GM media and ten - fold dilution of modified GM medial were tested in the isolation of halophilic and halotolerant bacteria with the 10% cruse salt as select pressure and using the selected media, the further isolation of 40 soil samples around Tarim basina was also conducted. The results showed that the cuhurable groups of halophilic and halotolerant bacteria in Tarim basin were oligotrophic and the ten -fold dilution of modified media obtained a best isolation results. The number of cuhurable halophilic and halotolarent bacteria ranged from 1000 to 100,000 cfu/g. And the total counts in different inhabits followed the sequence of crop filed 〉 desert soil 〉 bog sediment and plant rhizosphere 〉 plantation soil, and the numbers of different colony in the soil samples showed an order of crop filed 〉 bog sediment 〉 plantation soil and plant rhizosphere 〉 desert soil. The multivariate statistics results showed there was no significant correlationship between the bacteria counts and the property of soil samples such as nutrients and salt content, which suggested that the different groups of halophilc and halotolarent bacteria present in various inhabits.
出处
《塔里木大学学报》
2007年第2期53-56,70,共5页
Journal of Tarim University
基金
塔里木大学校长基金重点资助项目(编号:2004-01-02)
关键词
嗜盐及耐盐菌
塔里木盆地
halophilic and halotolerant bacteria
Tarim basin
