摘要
建立HPLC测定千金止带丸中芍药苷含量的方法。采用Reliasil—C18色谱柱，流动相为乙腈-水（12：88），流速为0．8mL·min^-1，检测波长为230m，柱温40℃。芍药苷在0．14～0．70μg范围内具有良好的线性关系，r=0．9999，平均回收率为99．1％，RSD=0．91％（n=5）。本法简便，准确，灵敏度高，重复性好，可用于该药品成分的含量测定。
A method for determination of Paeoniflorin in Qianjinzhidai pills by HPLC was established. The HPLC system consisted of Reliasil-C18 column, HCN- H2O （12 ： 88） mixture as a mobile phase, detection wavelength at 230nm, 0.8ml ·min^-1 of flow rate and column temperature at 40℃. The linear range of Paeoniflorin was 0. 14-0.70μg, r=0. 9999, the recovery was 99.09%, RSD=0. 91% （n=5）. The method is simple, the result is accurate and higher sensitivity, the reproducibility is good. This method can be used for the determination of Qianiinzhidai pills.
出处
《中国药事》
CAS
2007年第6期422-423,共2页
Chinese Pharmaceutical Affairs
