摘要
目的:建立雷公藤多苷中雷公藤内酯醇的含量测定方法。方法:雷公藤多苷以乙酸乙酯-丙酮(3:1)柱层析洗脱后,采用 HPLC 法进行测定。色谱柱:广州菲罗门科学仪器有限公司 Phenomenex C_(18)柱(250 mm×4.6 mm,5μm)。流动相A:0.05mol·L^(-1)磷酸二氢钠溶液(磷酸调 pH=4.0)-乙腈-甲醇(60:15:25);流动相 B:甲醇。梯度洗脱条件:0～18 min,A(100%),18～25 min,B(100%),25～30 min,A(100%)。流速:1.0 mL·min^(-1);检测波长为218 nm;柱温为15℃。结果:雷公藤内酯醇与其他成分分离良好。线性范围为0.44～14.16μg·mL^(-1!r=0.9999)。低中高浓度的平均回收率分别为95.4%,95.9%,96.6%。结论:方法简便、准确、重现性好,可用于雷公藤多苷的质量控制。
Objective： To develop an RP - HPLC method for the determination of triptolide in Tripterygium wilfordii polyglyeosides. Methods： The pretreatment was carried on a neutral alumina, eluted with ethyl acetate - acetone （ 3 ： 1 ）. Then,the gradient elution was used with a flow rate of 1.0 mL · min^-1 and UV detection at 218 nm. The column temperature was 15 ℃. The column was Phenomenex C18 column（250 mm ×4. 6 mm,5 μm） purchased from Guangzhou FLM Scientific Instrument Co. Ltd. Mobile phase A：0. 05 mol · L^-1 sodium dihydrogen phosphate（ adjusted to pH 4. 0 with phosphoric acid） - acetonitrile - methanol（60：15：25 ） ;Mobile phase B ：methanol. Condition of the gradient elution ：0 - 18 min, A （ 100% ） ; 18 - 25 min, B （ 100% ） ; 25 - 30 min, A （ 100% ）. Results： Triptolide was well separated. The linear calibration curve was obtained in the concentration range of 0. 44 - 14. 16 μg · mL^-1（r = 0. 9999）. The average recoveries of triptolide at low, medium and high concentrations were 95.4%, 95.9% and 96. 6% respectively. Conclusion ：The method is simple, accurate and reproducible. It is also proposed to be used for the quality control of Tripterygium wilfordii polyglyeosides.
出处
《药物分析杂志》
CAS
CSCD
北大核心
2007年第6期896-898,共3页
Chinese Journal of Pharmaceutical Analysis
基金
科技部"十五"重大科技专项(编号2001BA701A46)
关键词
雷公藤多苷
雷公藤内酯醇
HPLC
Tripterygium wilfordii polyglycosides
triptolide
HPLC
