摘要
以星花凤梨的短缩茎、老叶鞘、嫩叶鞘为接种外植体,对凤梨的离体培养技术进行了初步研究。结果表明,不同部位对愈伤组织诱导差异显著,其中老叶鞘诱导率最高,可达到53.3%,为最佳的取材部位。诱导愈伤组织培养基以MS+6-BA2.0 mg·L^(-1)+NAA 0.2 mg·L^(-1)为最好;MS+BA 2.0 mg·L^(-1)+N从0.25 mg·L^(-1)对不定芽分化效果良好。
Taking contraction stem, old leaf sheath, tender leaf sheath as inoculating explants, the tissue culture technology of Guzmania lingulata was studied. The results showed that： there was a significant difference in rates of callus induction for contraction stem old leaf sheath and tender leaf sheath, old leaf sheath was the best for callus induction with the rate of 53.3%. Medium for callus induction was MS ＋ 6-BA 2.0 mg · L^-1 ＋ NAA 0.2 mg · L^- 1 ; the optimum medium for induction was shoot MS ＋ 6 - BA 2.0 mg · L^- 1 ＋ NAA 0.25 mg · L^- 1.
出处
《西北林学院学报》
CSCD
北大核心
2007年第4期95-97,共3页
Journal of Northwest Forestry University
基金
陕西省苗木繁育中心项目"林果新品种引种与克隆"(14220304)
关键词
凤梨
愈伤组织
组织培养
快速繁殖
Guzmania lingulata
callus
tissue culture
rapid propagation
