摘要
目的：研究基质金属蛋白酶1（metal matrix proteinase-1，MMP-1）启动子区-1607bp处1G或2G单核苷酸多态性（single nucleotide epolymorphism，SNP）与贵州汉族人非小细胞肺癌（non—small cell lung carcinoma，NSCLC）遗传易感性的关系。方法：采用聚合酶链反应-限制性片段长度多态性（polymerase chain reaction—restriction fragment length polymorphism，PCR—RFLP）方法分析71名NSCLC患者和75名健康对照者的MMP-1启动子区SNP的基因型，比较不同基因型与NSCLC发生风险的关系。结果：2G／2G基因型在NSCLC组中分布频率高于健康对照组。2G／2G基因型较1G／1G及2G／2G＋1G／2G基因型患NSCLC风险率增加，OR（95％CI）值分别为3．082（1．620—5．862），2．517（1．283—4．936），吸烟可增加2G／2G基因型患NSCLC风险率。结论：贵州汉族人群MMP-1启动子区SNP（1G／2G）基因型与NSCLC易感性有关，2G／2G基因型的NSCLC发生风险增加。
Objective： To investigate the relation of the single nueleotide polymorphism （SNP） in matrix metalloproteinase 1 （MMP-1） gene with the susceptibility to non-small cell lung carcinoma （NSCLC） of Han nationality in Guizhou province. Methods： MMP-1-1607 （1 G-2G） of 71 NSCLC patients and 75 healthy people were amplified with polymerase chain reaction-restriction fragment length polymorphism analysis （PCR-RFLP） technique, and the polymorphism was analyzed. The relationship of different genotypes with the susceptibility of NSCLC was analyzed. Results： The distribution frequency of individuals with genotype 2G/2G was significantly higher in NSCLC patients than that in healthy controls. The individuals with genotype 2G/2G had higher risk in suffering from NSCLC than individuals with genotypes of 1G/2G or 2G/2G ＋ 1G/2G, and the OR values （95% CI） were 3. 082 （ 1. 620 -5. 862） and 2.517 （ 1. 283 -4. 936） respectively. Smoking could increase the risk of NSCLC. Conclusions： SNP of genotype （1G/2G） in promotor area of MMP-lgene in Han people of Guizhou relates to the susceptibility of NSCLC. People have genotype 2G/2G have higher risk to be attacked by NSCLC.
出处
《贵阳医学院学报》
CAS
2007年第3期267-269,272,共4页
Journal of Guiyang Medical College
关键词
基质金属蛋白酶
多态性
单核苷酸
癌
非小细胞肺
疾病遗传易感性
matrix metalloproteinases
polymorphism, single nucleotide
carcinoma, non-small-cell lung
genetic predisposition to disease
