摘要
东海原甲藻（Prorocentrum donghaiense Lu）是中国沿海频繁发生的大规模赤潮的原因种之一。文章对东海原甲藻的命名、生理生态和分子生物学等方面的研究成果作了综述。东海原甲藻与在日本、韩国等亚洲海域分布的被报道为Prorocentrum dentatum的原甲藻以及来自美国CCMP的具齿原甲藻（Prorocentrum dentatum）为同一种。东海原甲藻是一种喜长光照的赤潮藻类,其生长的最适温度为22℃,最适盐度为25-31。东海原甲藻能利用铵盐、硝酸盐和亚硝酸盐,最适生长的w（N）/w（P）比范围在8-20之间。作者构建了东海原甲藻cDNA文库并进行了EST分析,发现了22个与东海原甲藻生长发育、物质转换和能量代谢相关的基因标签。认为从基因表达及调控角度研究赤潮形成与消亡机制并阐明它与环境因子的关系是必要的。
As one of red tide causing species, Prorocentrum donghaiense has caused the largest and most frequent red tides in Eastern China Sea. In this paper the advances in identification, physiology, ecology and the moleculer biology ofP. donghaiense were reviewed. According to the molecular data of LSU rDNA, SSU rDNA and ITS, P. donghaiense and P.dentamm CCMP are the same species. P. donghaiense is a kind of Iong-photoperiod and photophilous algae. The optimal temperature is 22 ℃ and salinity ranges from 25 to 3 I. Three nitrogen sources （NaNO3, NH4Cl and NaNO2） can be utilized by P. donghaiense. The optimum w（N）/w（P） ratios for all three N sources are between 8 and 20. A cDNA library was constructed and EST analysis was carded out of this species. Twenty two ESTs were determined to be known genes and found to be involved in the biological processes including energy metabolism, material transformation and cell growth. It is useful to study the relationship between gene expression level and environmental factors to know how the bloom formed and crashed.
出处
《生态环境》
CSCD
北大核心
2007年第3期1053-1057,共5页
Ecology and Environmnet
基金
国家自然科学基金项目(40176028)
关键词
东海原甲藻
命名
生理生态
分子生物学
Prorocentrum donghaiense Lu
identification
physiology
ecology
moleculer biology
