摘要
本文根据系统分析方法，利用正交旋转组合设计于固体培养系统中测定培养基的装样量、细菌培养时间、接线虫量和线虫培养时间对Ｓｔｅｉｎｅｒｎｅｍａｃａｒｐｏｃａｐｓａｅ和Ｈｅｔｅｒｏｒｈａｂｄｉｔｉｓｂａｃｔｅｒｉｏｐｈｏｒａ产量的综合影响，确定线虫的优化培养参数。在设计组合范围内，接线虫量对Ａ２４线虫产量的影响显著；培养基的量和细菌培养时间对线虫产量的影响不显著。对Ｈ０６线虫产量，接线虫量、培养时间呈显著的正影响；细菌培养时间则呈显著的负影响。此外，接线虫量与培养时间对两种线虫均产生明显的负交互效应。培养基的装样量对线虫产量无显著影响。通过频数分析，分别得到了Ａ２４和Ｈ０６线虫的培养参数的优化组合。即每克培养基的Ａ２４线虫产量大于５０×１０４时，培养参数的优化组合是培养基的量为每瓶５３．６～５８．８克，细菌培养时间２天左右，接线虫量约每克培养基４．６×１０３，培养时间为１６～１８天。同理，每克培养基的Ｈ０６线虫产量大于２５×１０４时，培养参数的优化组合分别为每瓶培养基的量６７．８～７４．２克，细菌培养时间１～２天，接线虫量为每克培养基５．５×１０２～２．２×１０３之间，培养时间２２～２４天。根据这些培养参数的优化?
Effects or interactions of four culture parameters on the yield of Steinernema carpocapsae A24 and Heterorhabditis bacteriophora H06 were determined in solid cultures. Yields of infectives were significantly positively influenced by nematode inoculum size(ranging from 4.6￣4.6×10 4 for A24 and 5.5￣5.5×10 4 for H06 per g medium) and culture time of nematodes (ranging from 8￣24 days for A24 and 10￣30 days for H06) for both strains, and significantly negatively influenced by bacterial culture time(0￣4 days)for H06, but not by quantity of medium per flask (20￣100g/flask) for both strains or by bacterial culture time for A24. There were significant negative interactions between nematode inoculum size and culture time of nematodes on both strains. Optimum culture parameters were modeled for both strains and commercial operation in the culture system was suggested
出处
《昆虫天敌》
CSCD
1997年第2期75-83,共9页
Natural Enemies of Insects
基金
瑞典国际科学基金
关键词
昆虫病原线虫
固体培养
培养参数
Steinernema carpocapsae\ Herterorhabditis bacteriophora \ Mass production\ Solid culture\ Culture parameters
