摘要
经混酸处理后的多壁碳纳米管（MWCNTs）末端及侧壁带有含氧基团，能与阳离子聚电解质通过静电作用结合，也能与酶蛋白非特异性结合。利用层层自组装法在铂（Pt）电极表面构建了聚丙烯胺（PAA）-MWC-NTs-葡萄糖氧化酶（GOx）膜，研究了自组装薄膜的表面微观形貌和电化学性质。组装层数为6层时最优，对葡萄糖响应线性范围为5．0×10^-4 -2．10×10^-2mol／L；检出限为1．0×10^-4mol／L（S／N=3）；灵敏度为4.95μA／（mmol cm^2），响应时间3．80s；GOx表观米氏常数为17．79mmol。对抗坏血酸等具有较强的抗干扰能力，10天后电极响应电流保持最初的91．79％。3次平行实验的RSD为4．85％。
The terminals and side-walls were linked with oxygen-containing groups when muhiwalled carbon nanotubes （MWCNTs） were treated with concentrated acid mixtures. The groups can electrostatically interact with positively charged polyelectrolytes; can also non-specifically combine with enzymes. The layer-by-layer self-assembly approach was used to fabricate membrane on platinum （Pt） electrode using polyallylamine （ PAA）, MWCNTs and glucose oxidase （GOx）. The surface and electrochemical characteristic of self-assembled membrane were investigated. The optimum assembly number was 6. The linear range for glucose concentration was 5.0 × 10^ -4 -2.10 × 10^ -2 mol/L with a detection limit of 1.0×10^-4 mol/L （ S/N = 3 ）. The sensitivity was 4.95 μA mmol^-1 cm^-2 with a response time of 3.8 s. The apparent Michaelis-Menten constant （Km^app） of GOx was 17.79 mmol. The electrode had a good anti-interference ability to ascorbic acid etc.. It remained 91.8% of its initial current response after 10 days. The RSD was 4.9% after 3 paralleled detection.
出处
《分析化学》
SCIE
EI
CAS
CSCD
北大核心
2007年第7期1021-1024,共4页
Chinese Journal of Analytical Chemistry
基金
国家自然科学基金重大研究计划重点资助项目(No90409021)
关键词
多壁碳纳米管
聚丙烯胺
葡萄糖氧化酶
葡萄糖
层层自组装
生物传感器
Multiwalled carbon nanotubes, polyallylamine, glucose oxidase, glucose, layer-by-layer self- assembly, biosensor
