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软脉冲剂对mox-LDL引起的血管平滑肌细胞增殖的影响

Effect of Ruanmai Granulum on Proliferation of Bovine Aortic Smooth Muscle Cells Induced by Mildly Oxidized LDL
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摘要 目的:观察软脉冲剂对mox-LDL诱导血管平滑肌细胞(VSMC)增殖的影响。方法:培养的血管平滑肌细胞分为对照组、mox-LDL组和用药组(mox-LDL+不同剂量软脉冲剂),应用MTT法和流式细胞仪分别测定细胞含量和细胞周期,用硫代巴比妥酸比色法测定细胞培养液中丙二醛(MDA)的水平。结果:软脉冲剂能明显降低细胞培养液中MDA的水平,MTT检测显示软脉冲剂具有浓度依赖性抑制VSMC的增殖的的作用,流式细胞仪检测表明,用药组(S+G2M)期降低。结论:软脉冲剂能抑制mox-LDL刺激的SMC的增殖,并具有抗脂质过氧化作用。 Objective : To investigate the effect of Ruanmai Granulum on proliferation and lipid peroxldation oxidized of bovine vascular smooth muscle cells (VSMC). Method :The subjects were divided into three groups: control group, mildly Low Density Lipoprotein(mox- LDL) group and Ruanmai Granulum Treatment group who were treated with three different dosages. Using experimental models of proliferation of cultured bovine VSMC induced by mildly oxidized - low density lipoprotein , the level of MDA was determined with thiobarbituric acid reactive substance and the number of bovine VSMC was measured with MTT and the cell cycle phase was measured by a flow cytometer. Result :Ruanmai Granulum markedly reduced the level of MDA in the medium and inhibited MTT metabolism of VSMC. Ruanmai Granulum was shown to suppress VSMC proliferation in a concentration - dependent fashion. Ruanmai Granulum reduced the ( S + G2 M) block in cell cycle traverse. Conclusion : Ruanmai Granulum can inhibit the proliferation of VSMC. The inhibitory effect may be related to the inhibition of lipid peroxidation.
出处 《辽宁中医杂志》 CAS 北大核心 2007年第7期997-998,共2页 Liaoning Journal of Traditional Chinese Medicine
基金 江苏省科委社会发展基金资助项目(BS9521)
关键词 软脉冲剂 mox-LDL 平滑肌细胞增殖 丙二醛 Ruanmai granulum mox - LDL VSMC Proliferation MDA
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