摘要
为探讨卵泡刺激（ＦＳＨ）与黄体生成生素（ＬＨ）分泌及基因表达与调控用抗人ＬＨ－β亚单位单克隆抗体和抗人ＦＳＨ－β亚单位单克隆抗体进行免疫细胞化学定位的研究。通过连续切片免疫酶技术，免疫细胞化学双标记及电镜免疫金双标记技术，在大鼠垂体前叶进行ＦＳＨ和ＬＨ的免疫细胞化学共定位研究。结果表明：存在着棕（ＤＡＢ显色示ＦＳＨ）、蓝（ＣＮ显色示ＬＨ）及棕蓝混合染色细胞；电镜免疫金双标记发现分别被１５ｎｍ金粒（示ＦＳＨ）、１０ｎｍ（示ＬＨ）及被１５ｎｍ、１０ｎｍ金粒同时标记的３种细胞（另文报道）；光镜连续切片染色亦发现ＦＳＨ阳性、ＬＨ阳性及ＦＳＨ－ＬＨ双阳性３种细胞。因此可以确定垂体前叶存在３种促性腺激素（ＧＴＨ）细胞，即ＦＳＨ细胞（只含ＦＳＨ）、ＬＨ细胞（只含ＬＨ）和ＦＳＨ－ＬＨ细胞（既含ＦＳＨ又含ＬＨ）。
The cellular co localization of follicle stimulating hormone (FSH) and luteinizing hormone (LH)in the pituitary gland applying the anti β FSH/β LH McAb through the techniques of immunoenzyme on the serial paraffin sections and immunocytochemistry with double labelling had been proceeded.The results showed that there was a real co localization of FSH and LH on gonadotrophs: Three different kinds of cells could be identified by colour difference of immunostaining,these were brown ( FSH cell only).blue(LH cell only) and bluish brown (FSH LH cell) after utilizing of immuno double labelling. Meanwhile, three kinds of cells (only FSH positive staining cells, only LH positive staining cells and both FSH and LH positive staining cells) were distinguished after immunostaining on serial sections. Therefore, the conclution was obtained that there were three kinds of gonadotrophs in pituitary: FSH cells,LH cells and FSH LH cells. The possible reasons why exist these three types gene expression of gonadotrophic hormones in the anterior pituitary were discussed.
出处
《解剖学报》
CAS
CSCD
北大核心
1997年第1期53-58,共6页
Acta Anatomica Sinica
基金
国家自然科学基金
