摘要
目的建立高效液相色谱法测定两个不同产地的车前草中齐墩果酸的含量。方法采用C18柱,流动相是甲醇-水（85∶15）,流速：1.0 ml·min^-1;柱温：25℃。结果齐墩果酸在25-125 mg·L^-1的浓度下线性关系良好（r=0.999 7）,平均加样回收率分别为98.635%和98.99%。结论该法准确,快速、灵敏,重现性好。
Aim To determine the contents of oleanolic acid in Plantago asiatica L. yielded in differe regions by HPLC. Method Methanol-water（85： 15 ）was used as the mobile phase on C18 column, with a flow rate at 1.0 ml · min^-1, and temperature at 25 ℃. Result The linear range was 25 - 125mg ·L^-1 （ r = 0. 999 7 ） . The average recovery was 98. 635% and 98.99%, respectively. Conclusion The method is simple, sensitive, and highly reproductive.
出处
《安徽医药》
CAS
2007年第7期605-606,共2页
Anhui Medical and Pharmaceutical Journal
关键词
车前草
齐墩果酸
含量测定
plantago asiatica L.
oleanolic acid
content
