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循环伏安法研究天青A与ds DNA的相互作用 被引量:3

Studies on the Interaction of Azure A to dsDNA by Cyclic Voltammetry
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摘要 用循环伏安法研究了染料天青A(AA)与鲱鱼精DNA(dsDNA)在0.20 mol/L pH4.5的HAc-NaAc缓冲溶液中的相互作用。AA在玻碳电极上分别于-0.134 V和-0.082 V(vs.SCE)有一个还原峰和相应的氧化峰。加入dsDNA后,AA的还原峰和氧化峰电流均明显减小,但电子转移系数和电极反应标准速率常数基本不变。AA与dsDNA相互作用生成了一种非电活性的超分子复合物。求得AA与dsDNA复合物的结合比为n(AA)∶n(DNA)=3∶1,结合常数β=2.51×1015。紫外-可见吸收光谱法结果与循环伏安法结果一致。 A cyclic voltammetric investigation on the interaction of azure A (AA) to dsDNA in 0.20 mol/L HAc - NaAc buffer solution of pH 4. 5 was described. AA yields a well-defined reduction peak at -0. 134 V and a well-defined anodic peak at -0. 082 V on the glassy carbon electrode. After the addition of dsDNA, the currents of both the reduction peak and the anodic peak decrease dramatically, whereas the electrochemical parameters( such as the electron transfer coefficient a and the standard rate constant k^s of the electrode reaction) have no significant changes. The interaction of AA to dsDNA forms an electrochemically inactive supramolecular complex dsDNA : mAA. The composition of the complex was determined to be n(AA) : n(dsDNA) =3 : 1, with the binding constantβ =2. 51 x 10^15. The experi- mental results of ultraviolet -visible spectroscopy are consistent with the cyclic voltammetry.
出处 《分析测试学报》 CAS CSCD 北大核心 2007年第4期492-495,共4页 Journal of Instrumental Analysis
基金 国家自然科学基金资助项目(20375020) 青岛市自然科学基金资助项目(04-2-JZP-8)
关键词 DNA 天青A 循环伏安法 电化学 吸收光谱法 DNA Azure A Cyclic voltammetry Electrochemisty Absorption spectroscopy
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  • 1张朝红,臧树良,苏欣,耿兵,赵迪,冯冲,董殿波.三苯基锡化合物与DNA作用的光谱研究[J].分析测试学报,2005,24(3):63-66. 被引量:8
  • 2ERDEM A, KERMAN B, MERIC U S, et al. Novel hybridization label methylene blue for the electrochemical detection of short DNA sequences related to the hepatitis B virus[J]. Anal Chim Acta, 2000, 422(2) : 139 - 149.
  • 3ERDEM A, OZSOZ M. Interaction of the anticancer drug epirubicin with DNA[J]. Anal Chim Acta, 2001,437( 1 ) : 107- 114.
  • 4ERDEM A, OZSOZ M. Vohammetry of the anticancer drug mitoxantrone with DNA[J]. Turk J Chem, 2001,25 : 469 -475.
  • 5JAMIESON E R, LIPPARD S J. Structure, recognition and processing of cisplatin - DNA adducts[ J]. Chem Rev, 1999,99 (9) : 2467 - 2498.
  • 6TANI A, TOMSON A J, BUTT J N. Methylene blue as an electrochemical discriminator of single- and double-stranded oligonucleotides immobilized on gold substrates[J]. Analyst, 2001, 126: 1756- 1759.
  • 7ROHS R, SKLENAR H, LAVERY R, et al. Methylene blue binding to DNA with alternating GC base sequence: a modeling study[J]. J Am Chem Soc, 2000, 122(18) : 2860 -2866.
  • 8ZHAO G, ZHU J, CHEN H. Spectroscopic studies of the interactive model of methylene blue with DNA by means of β-cyclodextrin[J]. Spectrochim Acta A, 1999, 55:1109 -1117.
  • 9OZKAN D, KARA P, KERMAN K, et al. DNA and PNA sensing on mercury and carbon electrode by using methylene blue as an electrochemicallabel[ J]. Bioelectrochemistry, 2002, 58( 1 ) : 119 - 126.
  • 10KARA P, KERMAN K, OZKAN D. Electrochemical genosensor for the detection of interaction between methylene blue and DNA [ J ]. Electrochemistry Communications, 2002, 4 (9) : 705 - 709.

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