摘要
目的探讨甲胎蛋白异质体（AFP-L3）在肝细胞癌诊断中的意义。方法应用预装有耦联小扁豆凝集素（LCA）的琼脂糖微量离心柱分离AFP-L3，采用双抗体夹心、化学发光法检测AFP和AFP-L3，并计算AFP—L3值，以AFP〉20μg／L，AFP-L3〉10％作为阳性判断标准。结果肝细胞癌患者血清中AFP、AFP-L3水平明显高于其他肝病患者，P值均〈0．001，肝癌患者以AFP、AFP-L3作为诊断指标的敏感度分别是85．1％、72．3％，特异度分别是55．4％、97．2％；8例肝病患者AFP-L3〉10％，影像诊断未发现肝癌，至少三个月以后确诊肝癌。结论甲胎蛋白异质体（AFP—L3）在肝细胞癌诊断及与良性肝病的鉴别诊断中具有重要价值。
Objective To evaluate the chnical significance of AFP-L3 in the patients with hepatoceUular carcinoma and other chronic hepatitis, Method Serum AFP-L3 variants were separated by micro centrifugal column, and detected by chemiluminescence, Results AFP and AFP-L3 levels were higher in hepatoceUular carcinoma than that in other chronic hepatitis （ P 〈 0.001 ） ; as a diagnosis target, the sensitivity and specificity of AFP-L3 were 72,3 % and 97.2 % . Eight patients with hepatitis have higher AFP-L3, but none of then were found carcinoma by CT three months later. Conclusion AFP-L3 is very useful in the diagnosis of patients with hepatoeeUular carcinoma.
出处
《中华实验和临床病毒学杂志》
CAS
CSCD
北大核心
2007年第2期135-137,共3页
Chinese Journal of Experimental and Clinical Virology
关键词
甲胎蛋白类
癌
肝细胞
诊断
alpha-fetoproteins
Carcinoma, hepatocellular
Diagnosis
