摘要
目的建立人禽流感H5抗体的酶联免疫检测方法（ELISA）。方法用ELISA法检测疑似高致病性禽流感患者及正常人群血清标本中H5IgG抗体。结果23例疑似高致病性禽流感患者血清样品中有4例H5IsG抗体阳性，阳性率为17．40％，与血凝抑制试验（HI）和微量中和试验（NI）相比较，三者符和率为100％。234例正常人群血清H5 IgG抗体检测均为阴性。结论建立的ELISA方法可用于高致病性人禽流感的血清样品初步筛查。
Objective To establish a convenient method for diagnosis of lethal avian influenza virus infection. Methods Ninety-six-well microtiter plates were coated with 100μl of HSN1 protein （0.1μg/ml） diluted in coating buffer. These plates were used for enzyme-linked immunosorbent assay （ELISA）. Results Four of the 23 suspected borderline cases of lethal avian influenza virus infection were positive by the ELISA, and all the healthy controls （n = 234） were negative. These results were confirmed by hemagglutination-inhibition （HI） and neutralization tests, and results were totally consistent （100%） with each other among the three methods. Conclusion The ELISA may be used in the screening for lethal avian influenza virus infection.
出处
《中华实验和临床病毒学杂志》
CAS
CSCD
北大核心
2007年第2期180-181,共2页
Chinese Journal of Experimental and Clinical Virology
关键词
人禽流感
免疫球蛋白G
酶联免疫吸附测定
Avian influenza
Immunoglobulin G
Enzyme-linked immunosorbent assay
