摘要
利用电子显微技术研究了丙烷脒在活体植物上对番茄灰霉病菌Botrytis cinerea Pers.生长发育的影响。结果表明:丙烷脒对番茄灰霉病菌的生长发育有明显影响,但不同时期施药,表现出一定的差异性。扫描电镜观察发现,在先施药后接菌的情况下,番茄灰霉病菌产孢量显著下降,并且所产孢子表现出凹陷、变形等不良发育状况;在先接菌后施药的情况下,丙烷脒处理后不影响番茄灰霉病菌的产孢量,但所产孢子也表现出凹陷、变形等不良发育状况;透射电镜观察发现,在先施药后接菌的情况下,番茄灰霉病菌无法有效地侵入番茄表皮以下组织,病情得以有效控制;在先接菌后施药的情况下,药剂处理的番茄灰霉病菌可以侵入番茄表皮以下组织,但生长状况不良。不同时期丙烷脒处理后均能使番茄灰霉病菌的菌丝出现塌陷、生长点缢缩等不良生长现象;病菌菌丝细胞壁表现出不规则的加厚,尤以顶端加厚明显;线粒体异常增多,并出现基质型肿胀;病菌菌丝内细胞器液泡化,最终形成大的液泡;病菌细胞内含物外渗,出现质壁分离和空腔细胞;病菌细胞器泡囊化解体和细胞组织崩解,细胞趋于死亡等。研究表明,丙烷脒对番茄灰霉病菌的生长发育具有明显的影响,除具有保护性防病效果外,还表现出较强的治疗效果。
The effect of propamidine on growth and development of Botrytis cinerea Pers. on living plants were examined using electron microscopy techniques. The studied revealed that propamidine have distinct influence on growth and development of Botrytis cirterea Pers. , but there were some difference when sprayed propamidine at various periods. Scanning electron micrographs revealed that the quantity of conidium decline and conidia collapsed practice of propamidine at the beginning of infestation; don＇ t influence the quantity of conidium but conidia collapsed practice of propamidine after infestation. Transmission electron micrographs revealed that Botrytis cinerea Pers. can＇ t invade tomato of epidermal organise practice of propamidine at the beginning of infestation ; but Botrytis cinerea Pers. can invade tomato of epidermal organise collapsed practice of propamidine after infestation. Propamidine can inhibited the hyphal growth, the tip of the hyphae were shrunken, considerable thickening of the hyphal cell walls, particularly at the hyphal tip region, cytoplasm inhomogeneity and inclusion in cell exosmosis, mitochondrion manifold, vesiculated hypha cell, cell empty and so on when sprayed propamidine at various periods. The cytological change resulted in the necrosis of hyphal cell. The studied showed that propamidine had distinct protective and therapeutic action influence.
出处
《植物保护学报》
CAS
CSCD
北大核心
2007年第3期305-310,共6页
Journal of Plant Protection
基金
国家"十五"科技攻关项目(2002BA516A04)
农业科技成果转化资金项目(04EFN216100284)
