摘要
利用GUS报告基因,建立了农杆菌介导的谷子(Setaria italica)遗传转化体系,并研究了多种影响因素对转化效率的影响,包括受体基因型、外植体类型、菌液浓度、培养基中乙酰丁香酮的浓度、侵染时间和共培养时间.确定了最优的转化条件为:以谷子幼穗诱导的愈伤组织为外植体,用低浓度的农杆菌菌液侵染30～40min,然后在含有0.1mmol/L乙酰丁香酮的LS培养基上共培养2d.
An efficient and repeatable approach in transforming the foxtail millet （Setaria italica ） using Agrobacterium LBA4404 horboring the plasmid pBI121 was established. Factors affecting transformation efficiency were investigated including the genotype, explants, the density of bacteria, the duration for inoculation and co-cultivation, and the concentration of acetosyringone in the medium. The maximum transformation conditions were：the callus induced from inflorescence was used as explant; the duration for inoculation with Agrobacterium at low cell density was 30-40 min; for the co-cultivation , the suitable concentration of acetosyringone in the medium was 0.1 mmol/L, and the duration was 2 days.
出处
《中国生物化学与分子生物学报》
CAS
CSCD
北大核心
2007年第7期531-536,共6页
Chinese Journal of Biochemistry and Molecular Biology
基金
国家转基因植物研究与产业化专项基金项目(No.JA03-A-16)~~
关键词
谷子
转化效率
农杆菌介导转化
影响因素
foxtail millet
transformation frequency
Agrobacterium-mediated transformation
influence factors
