摘要
从安徽某煤矿的酸性矿坑水中分离出能有效浸出低品位磷矿的氧化亚铁硫杆菌菌株,对其形态特征和生长特性进行了初步的研究,并通过研究不同固体培养基成分对氧化亚铁硫杆菌分离纯化的影响,确定了适宜的分离条件:9K固体培养基的Fe2+浓度为4.5g/L,采用下层涂布异养菌(红酵母菌)、上层涂布氧化亚铁硫杆菌的双层平板.考察了培养基、能源物质及表面活性剂等因素对所选育的氧化亚铁硫杆菌的浸磷效果的影响,通过初步实验,浸磷率可达48%.
A strain of Acidithiobacillusferrooxidans that can be used effectively in the leaching of low grade phosphate ore was isolated from acid mine drainage from a coal mine in Anhui Province in China. Its characteristics of morphology and growth were studied. Through the investigation of the effects of different solid culture medium components on the isolation and purification of Acidthiobacillus ferrooxidans, the optimum isolation conditions were determined as： the concentration of Fe^2＋ in 9K solid culture medium was 4.5 g/L. A double-layer plate, with heterotrophic microbe Rhodotorula sp. spreading onto the bottom layer and chemoautotrophic bacterium At. fspreading onto the upper layer, was used. The influential factors including culture media, energy source materials and the addition of surfactant on the leaching of phosphate ore with the cultivated At. fwere studied. Through preliminary experiments, up to 48% of phosphate in the ore was leached out using the isolated At.f strain.
出处
《过程工程学报》
EI
CAS
CSCD
北大核心
2007年第3期584-588,共5页
The Chinese Journal of Process Engineering
基金
国家重点基础研究发展规划(973)基金资助项目(编号:2004CB619204)
关键词
氧化亚铁硫杆菌
分离纯化
固体培养基
磷矿分解
细菌浸出
浸磷率
Acidithiobacillus ferrooxidans
isolation and purification
solid culture medium
phosphate ore dissolution
bacteria/ leaching
phosphate leaching rate
