摘要
目的研究曲古菌素A（TSA）在胃腺癌细胞系SGC-7901中的作用。方法四甲基偶氮唑蓝法测定TSA对SGC-7901的细胞毒性，荧光显微镜观察细胞核DNA的变化，流式细胞仪检测细胞周期和凋亡率，Western印迹检测组蛋白H3的乙酰化状态。结果SGC-7901细胞生长曲线表明，TSA浓度增高，细胞生长率下降。细胞凋亡率在75ng／mlTSA作用72h时与对照组比较，差异有统计学意义（P〈0．05）。凋亡细胞DNA变化主要表现为核染色质固缩及断裂，荧光染色增强。流式细胞仪分析出现明显的亚G1期峰，G1期细胞减少，细胞凋亡率达29．54％。TSA作用后，乙酰化的组蛋白如明显增多。结论TSA可以诱导SGC-7901细胞发生凋亡，组蛋白H3的乙酰化状态可能与细胞凋亡有关。
Objective To investigate the effect of trichostatin A （TSA） on SGC-7901 cells. Methods Cytotoxicity and cell viability of gastric cancer cell line SGC-7901 were assayed by MTT method. Morphologic assessment of apoptosis was performed with fluorescence microscope. Cell cycle and apoptosis rate were analyzed by flow cytometry. Histone H3 acetylation was detected by Western blot. Results TSA showed apparently cytotoxicity in SGC-7901 cells. The growth curve showed the growth ratio decreased with the increase of TSA concentration. Apoptosis rate were significantly different between TSA treated group（75 ng/ml for 72 h）and control group （P 〈 0.05）. Morphologic changes of apoptosis including nuclear chromatin condensation and fluorescence strength were observed with fluorescence microscope.TSA treatment （75 ng/ml for 72 h） sensitively induced apoptosis in the cell,which was demonstrated by the migration of many cells to the sub-Gl phase,the reduction of G1- phase cells and the increament of apoptosis rate （29.54%） in flow cytometric analysis. The expression of acetylated histone H3 was increased in TSA group （75 ng/ml） for 48 h compared with control group by Western blot. Conchisions TSA can induce SGC-7901 cell apoptosis. The expression of acetylated histone H3 may contribute to the apoptosis.
出处
《中华胃肠外科杂志》
CAS
2007年第4期376-379,共4页
Chinese Journal of Gastrointestinal Surgery
