摘要
目的探索测定胃康灵胶囊中延胡索乙素含量的方法。方法高效液相色谱法，色谱柱为C18柱，VP—ODS 150mm×4．6mm。流动相为甲醇-7％乙酸-4％乙酸铵（36：32：33），流速1．0mL·min^-1，柱温为25℃，检测波长为280nm。结果精密度和稳定性均良好；延胡索乙素浓度在0．032-0．177mg·mL^-1范围内，峰面积与浓度呈良好的线性关系，r=0．9997，平均回收率为98．4％；延胡索乙素与其相邻杂质峰的分离度符合要求。结论本方法简便、专属、重现性好。也可用于延胡索药材及其它制刺的含量测定。
OBJECTIVE To develop a method for determination of tetrahydropalmtine in weikangling capsules. METHODS Using a RP-HPLC method with C18 column,VP-ODS 150mm×4. 6mm, the mobile phase was a mixture of methanol-7% acetic acid-4%ammonium acetate （36 ： 32 ： 33）,flow rate was 1.0mL ·min^-1,column temperature was 25℃ and with the detection wavelength was 280 nm. RESULTS The precision and stability were fine. The linear correlation was observed for the concentration of 0. 032mg ·min^-1-0. 177mg·min^-1（ r = 0. 9997）. The average recovery was 98.4%. resolution between tetrahydropalmatine and other peaks met the requirements. CONCLUSION The method was convenient, selective and reproducible for determination of tetrahydropalmatine in Rhizoma Corydalis and its preparation.
出处
《齐鲁药事》
2007年第7期411-412,共2页
qilu pharmaceutical affairs
