摘要
目的从骨髓间质干细胞（MSCs）水平探讨RANKL及OPG与青少年特发性脊柱侧凸（AIS）患者骨量降低的关系。方法46例12-17岁志愿者分为2组,其中AIS组30例,年龄12-17岁,平均14.1岁。正常对照组16例,年龄12-17岁,平均14.7岁。AIS组Lenke1型14例,2型2例,3型6例,5型8例。2组均采用双能X线吸收测量仪测量BMD,测量部位包括非优势侧股骨近端及腰椎。分别从2组志愿者髂前上棘处骨穿刺抽取10ml骨髓,肝素抗凝。体外密度梯度离心法分离培养MSCs,扩增至P3代行表型鉴定,RT-PCR法检测2组MSCs中RANKL及OPG的 mRNA表达强度。结果AIS组腰椎（L2-L4）及股骨近端的BMD值明显低于正常对照组。AIS组RANKL的 mRNA表达显著强于对照组（P〈0.01）,而OPG的 mRNA表达显著低于对照组（P〈0.01）。结论RANKL及OPG在MSCs水平表达强度的异常可能与AIS骨量降低的分子机制相关。
Objective To investigate the relationship between RANKL/OPG and the decreased bone mass of adolescent idiopathic scoliosis patients at the MSCs level. Methods This study covered 46 subjects （aged from 12 to 17）, including patients with AIS and normal age-matched non-scoliosis children. AIS group was 30 patients, Normal group was 16 cases. The average Cobb angle of AIS group was 52.7°（ranging from 40°-105°）. One spine surgeon was responsible for carrying out physical examination of normal control patients and ruling out the possibility of scoliosis, growth disorders and other chronic diseases. BMD of the lumbar spine and proximal femur was measured using dual energy X-ray absorptiometry in two groups. 10ml of the human bone marrow was obtained from anterior superior iliac spine under the sterile conditions, then anticoagulated by calparine, and the hMSCs were isolated by density gradient centrifuge from the mononuclear cells, and then were cultivated and subcuhured in vitro. P3 generation was analyzed by the flow cytometry to detect the surface antigens, whereafter expressive intensity of RANKL and OPG of hMSCs from two groups were detected by using RT-PCR. Results There were negative expression in hMSCs of CD45-FITC,CD14-PE,CD34-PE and HLA-DR-PE, and then positive expression in that of CD29-FITC,CD44-FITC,CD105-PE both 2 groups. BMD of the lumbar spine （L2-L4） and proximal femur was obviously lower in AIS group than that of the control group. Expression of RANKL of MSCs had increased obviously in AIS group as compared with the control group（ P 〈 0.01 ）. On the contrary, was the expression of OPG, Conclusion The abnormal expression of RANKL and OPG of hMSCs may be responsible for the molecule mechanism of the decreased bone mass of adolescent idiopathic scoliosis patients.
出处
《中国骨质疏松杂志》
CAS
CSCD
2007年第7期474-479,485,共7页
Chinese Journal of Osteoporosis
基金
国家自然科学基金资助项目(30672131)
