摘要
目的研究黄芩茎叶总黄酮（TF）对人类单核肿瘤细胞U937增殖的影响。方法U937细胞以5×10^4个／ml的浓度，培养于含胎牛血清（FCS）10％的RPMI 1640全培养液中，TF浓度分别为0、25、50、100、150、2130mg／L组，每组8孔接种于24孔培养板中孵育7d。每天计数各孔细胞密度，第7天测定细胞悬液中乳酸脱氢酶（LDH）活性。结果TF 25～200mg／L组U937细胞密度均低于TF 0mg／L组，浓度越高细胞密度越低（P〈0．05）。TF 0～150mg／L组细胞悬液LDL活性差别无统计学意义（P〉0．05），TF 200mg／L组LDL与其他5组相比均具有统计学意义（均P〈0．05）。结论TF具有抑制U937细胞增殖的作用，高浓度的TF（〉1200mg／L）对体外培养的U937细胞具有毒性作用。
Objective To study the effects of total flavone of scutellaria＇s stem and leaf （TF） on U937 cell proliferation. Methods 48 holes of U937 cells （5 × 10^4/ml） cultured in RPMI1640 with 10% fetus cattle serum （FCS） were divided into 6 groups according to the concentration of TF （0 mg/L ,25 mg/L,50 mg/L, 100 mg/L, 150 mg/L,200 mg/L）. Every group was incubated for 7 days. Cell counting was applied every day and the activity of lactate dehydrogenase （LDH） in culture medium was measured on the 7th day. Results The density of U937 cells in TF 25 - 200 mg/L groups was significantly lower than that in TF 0 mg/L group （ P 〈 0.05 ）. The activity of LDH in TF 0 - 150 mg/L group had no significant difference （ P 〉 0.05 ） , and that in 200 mg/L group was higher than other groups （ P 〈 0.05 ）. Conclusion TF inhibits proliferation of U937 cells. TF probably poisons U937 cells with the concentration higher than 200 mg/L.
出处
《中国综合临床》
北大核心
2007年第9期801-802,共2页
Clinical Medicine of China
关键词
黄芩茎叶总黄酮
U937细胞
乳酸脱氢酶
Total flavone of scutellaria＇s stem and leaf
U937 cells
Lactate dehydrogenase
