摘要
目的研究不同剂量维生素E(VitE)对大鼠局灶性脑缺血再灌注后梗死周围区生长相关蛋白-43(GAP-43)及突触素P38表达的影响,从而进一步探讨抗氧化剂在脑缺血损伤中的作用机制及VitE应用剂量。方法成年健康雌性Wistar大鼠125只,随机分成假手术组、对照组、大剂量VitE组(300 mg/kg)、中剂量VitE组(50 mg/kg)和小剂量VitE组(2.5 mg/kg)。采用线栓法建立大脑中动脉缺血再灌注(MCAO)动物模型。各组分别于术后6 h、1 d、3 d、7 d、14d取脑片。应用免疫组化方法观察脑缺血再灌注后GAP-43和P38的表达。结果(1)小剂量VitE组GAP-43和P38表达较对照组有所增高,但差异无显著性(P>0.05)。(2)大、中剂量VitE组间GAP-43和P38表达比较未见明显变化(P>0.05)。(3)大、中剂量VitE组与对照组比较:GAP-43表达增高,1 d、3 d、7 d、14 d各时间点差异均有显著性(P<0.05);P38表达也增高,3 d、7 d、14 d时间点差异具有显著性(P<0.05)。结论抗氧化剂VitE可增强中枢神经系统损伤后神经元再生重塑功能。对于VitE最佳剂量的选择,尚有待进一步研究。
Objective To study the effect of different doses of Vitamin E onexpressions of growth associated protein-43 （GAP-43） and synaptophysin（P38）at damaged side after focal cerebral ischemic reperfusion, and to explore their roles in cerebral ischemia and reperfnsion injury in rats and the relationship between doses of Vitamin E and the effects were studied. Methods One hundred and twenty-five aduh female rats were divided randomly into sham-operation group, controlled group, high-dose VitE group（300 mg/kg, HVE）, middle-dose VitE group（50 mg/kg, MVE）and low-dose VitE group（2.5 mg/kg, LVE）. The focal cerebral ischemia/reperfusion models of rats were received right middle cerebral artery occlusion （MCAO）. The sections of the brains were obtained and expressions of GAP-43 and P38 with immtmochemistry were analyzed at 6 hours and 1,3,7,14 days after reperfusion. Results （1） The expressions of Ref-1, GAP-43 and P38 were slightly increased after low-dose VitE treatment, but there were no difference in above indexes between LVE group and controlled group（ P 〉 0.05）. （2） The differences were not remarkable in the expression of Ref-1 ,GAP-43 and P38 between HVE group and MVE group （P 〉 0.05）. （3） Compared high-dose and low-dose VitE treatment groups with controlled groups,GAP-43 immtmocompetence were elevated and the differences were remarkable at 1,3,7 and 14 days（P 〈 0.05） ; the expression of P38 were increased and the differences were remarkable at 3,7,14 days（ P 〈 0.05）. Conclusion This study suggests vitamin E, as an antioxidant, might be accelerate neuronal plasticity and reconstruction in central nervous system after reperfnsion in- jury. But the optimum dosage of vitamin E needs further exploration.
出处
《中国实验诊断学》
2007年第7期892-896,共5页
Chinese Journal of Laboratory Diagnosis
基金
黑龙江省教委资助项目(项目批准号9551118)
关键词
维生素E
脑缺血再灌注
生长相关蛋白
突触素
Vitamin E： cerebral ischemia/reoeffnsion- redox factor-1 （ Ref- 1 ） ： growth associated protein （GAP） ： svnantoDhvsin
