摘要
目的研究AmpC酶和ESBLs在临床分离铜绿假单胞菌中的分布及其对耐药性的影响。方法纸片扩散法测定19种抗生素的耐药性,三维试验检测高产AmpC酶和超广谱β-内酰胺酶(ESBLs)。结果92株铜绿假单胞菌中,单纯高产AmpC酶者,单纯产ESBLs者,高产AmpC酶并产ESBLs者分别为17.4%、8.7%、10.7%。产酶菌株仅对美洛培南亚胺培南敏感,对大多数抗生素耐药。非产酶菌株对头孢西丁、氨苄西林/舒巴坦、头孢噻肟、头孢唑林高度耐药,对大多数抗生素敏感。结论产ESBLs和高产AmpC酶的铜绿假单胞菌在临床分离菌株中阳性率较高,产酶菌株对大多数抗生素的耐药性明显高于非产酶菌株。
Objective To investigate the prevalence of AmpC β-lactamases and extended-spectrumβ-lactamases in Pseudomonas aeruginosa srain and its impact on antibiotic susceptibility. Methods The susceptibility study was tested using the disc diffusion method according to recommendation of the Clinical and Laboratory Standards Institute. A modified three-dimensional extract method was used to determine the phenotype of Pscudomonas aeruginosa harboring AmpC β-lactamases and extended-spectmnβ-lactamases. Results Among 106 strains of Pseudomonas aeruginosa, 17.4% produced only AmpC β-lactamases 8.7 % produced only ESBLs 10. 7% produced both high-level AmpC β-lactamases and ESBLs. Lactamase producing strains were only susceptible to meropenem, imipenem.non-lactamase producing strains were susceptible to most antibiotics. Conclusion The incidence of lactamases in Pseudomonas aeruginosa was rather high, lactamase producing strains show more resistance to most antibiotics than non-lactamase producing strains.
出处
《中国实验诊断学》
2007年第7期946-948,共3页
Chinese Journal of Laboratory Diagnosis
