摘要
为探索郁金香的快繁方法,并为转基因分子水平研究奠定基础,对4个郁金香品种‘Parade’,‘Merry Widow’,‘Golden Parade’,‘Pink Diamond’的鳞片组织培养进行系统的研究。结果表明:0.1%HgCl2 10min+5%NaClO 20min是最佳的消毒方法。‘Parade’适宜诱导愈伤组织的培养基为MS+NAA1.0mg·L-1+BA1.0mg·L-1,诱导率可达100%。‘Parade’和‘Merry Widow’适宜诱导芽的培养基分别为MS+NAA2.0mg·L-1+BA0.5mg·L-1和MS+NAA0.5mg·L-1+BA1.0mg·L-1,芽的直接再生率均为15.4%。中层鳞片易诱导成愈伤组织,诱导率为8.0%,内层鳞片较易直接诱导生芽,再生率可达26.7%。培养基中添加50～200mg·L-1的维生素C有利于直接再生芽,而不利于愈伤组织的诱导。鳞片接种后暗处理5d和15d可以使芽的直接再生率分别达到12.5%和10.3%。
The experiment was to look for a mothod of mieropropagation and establish a base for research of molecule level. The effect of six factors on tissue cuhure of bulb scales of four tulip cultivars was studied. The results showed that bulb sterilization with 0.1%HgC12 for ten minutes and 5% NaC10 for twenty minutes was the best. The best culture mediun for callus inducement of cv.‘Parade＇ was MS＋NAA1.0mg· L^-1 BA1.0mg·L^-1. The rate of callus inducement was up to 100%. The best culture media for shoot regeneration of cv. ‘Parade＇ and cv.‘Merry Widow＇ were MS＋NAA2.0mg·L^-1＋BA0.5mg·L^-1 and MS＋NAA0.5mg·L^-1＋ BA1.0mg·L^-1, respectively. The rates of shoot regeneration were all 15.4%. The middle-layer scales of bulb were easier to induce callus, with the rate 8.0%, but inner-layer scales of bulb were easier to induce shoots directly, with the rate 26.7%. Adding 50- 200mg·L^-1 ascorbie acid in culture medium was proved to be advantageous to the shoot regeneration, but to be disadvantageous to the callus inducement. The rates of shoot regeneration were up to 12.5% and 10.3% when bulb scales were given darkness treatments for five days and fifteen days after inoculating.
出处
《沈阳农业大学学报》
CAS
CSCD
北大核心
2007年第3期304-307,共4页
Journal of Shenyang Agricultural University
关键词
郁金香
鳞片
组织培养
tulip
bulb scale
tissue culture
