摘要
将非洲爪蟾蝌蚪从发育的第52期开始暴露在不同浓度(0、10、25、50、100μg/L)的多氯联苯(Aroclor1254)中,直到变态结束,研究其对非洲爪蟾变态发育的影响。结果表明,在变态高峰期前,对照组和试验组的存活率无显著的差别,而在变态高峰期,浓度高于10μg/L的试验组蝌蚪大量死亡,各组存活率分别为25μg/L组72.9%,50μg/L组53.1%,100μg/L组12.3%;各浓度组蝌蚪在变态结束时的体重无显著差异(P>0.05);50μg/L组和100μg/L组蝌蚪变态高峰期所需的时间与对照组相比差异显著(P<0.05);25μg/L以上浓度组,蝌蚪甲状腺组织表现不同程度的滤泡扩张,滤泡上皮细胞增生、叠加,滤泡数目增多等。提示多氯联苯可干扰非洲爪蟾的变态发育,两栖类变态试验可以作为筛选甲状腺激素干扰物的一种简便可行的方法。
Thyroid hormone disruptor is an important group of endocrine disruptors. In this test, Xenopus laevis tadpoles were exposed to Aroelor1254 at gradient concentration （0, 10, 25, 50, 50 μg/L） from stage 52 to metamorphosis completion. The effects of Aroclor1254 on the developmental by metamorphosis of Xenopus laevis were observed. The results showed that the time completing metamorphosis of Xenopus laevis exposed at higher concentration （50 μg/L, 100 μg/L） was delayed. The thyroid histology of Xenopus laevis tadpoles changed at concentrations over 25μg/L. The extent of thyroid follicle dilatation, the follieulor cell hyperplasia, and the folliculor cell height were strengthened with the exposure concentration increased. These results indicated that polyehlorinated biphenyls can disrupt the metamorphosis of Xenopus laevis, and induce the histological change of thyroid gland. The amphibian metamorphosis assay is a simpler and sensitive assay in screening thyroid hormone disruptor.
出处
《动物医学进展》
CSCD
2007年第7期1-6,共6页
Progress In Veterinary Medicine
基金
国家自然科学基金项目(20677074)
关键词
多氯联苯
非洲爪蟾
变态
内分泌干扰物
甲状腺激素干扰物
polyehlorinated biphenyls
Xenopus laevis
metamorphosis
endocrine disruptor
thyroid hormone disruptor
