摘要
目的确定大肠癌新相关基因ＨＳＵ１７７１４的染色体定位。方法采用强化荧光原位杂交技术（ＦＩＳＨ），以生物素化酪胺强化荧光原位杂交信号。结果８０．０％（１２８／１６０）的间期细胞和５９．８％（１０４／１７４）的中期分裂相可见到明显集中的ＨＳＵ１７７１４基因的杂交信号，相应荧光Ｒ带分析中，８５．１％（４０／４７）在２２号染色体上１区３带处有杂交信号。结论ＨＳＵ１７７１４基因定位于２２ｑ１３。
Objective To identify the chromosomal localization of novel colorectal cancer related gene HSU17714.Methods Enhanced fluorescence in situ hybridization technique was applied with tyramine as the enhancer.Results 80%(128/160) interphase and 60% (104/174) metaphase cells showed obvious hybridization signals of HSU17714.85%(40/47) metaphase cells showed hybridization signals at 22q13 in the fluorescence R banding assay.Conclusion HSU17714 is assigned to human chromosome 22q13.
出处
《中华肿瘤杂志》
CAS
CSCD
北大核心
1997年第3期177-179,共3页
Chinese Journal of Oncology
关键词
癌基因
染色体
原位杂交
大肠肿瘤
Colorectal neoplasms Oncogenes Chromosome In situ hybridization
