摘要
目的探讨维拉帕米对实验性急性胰腺炎的保护作用．方法雄性Ｗｉｓｔａｒ大鼠２２只，随机分为假手术组（ｎ＝５）、急性胰腺炎组（ｎ＝７）和维拉帕米保护组（ｎ＝１０）．用２０％牛胆酸钠溶液０１ｍｌ／１００ｇ逆行注入大鼠胰管内诱导急性胰腺炎．保护组在实验前经颈静脉输注维拉帕米１０ｍｇ／ｋｇ·ｈ维持１２ｈ．在０，６及１２ｈ分别断尾取血１０ｍｌ，测定血清淀粉酶活性．同时，在光镜和电镜下观察各组胰组织的病理变化．结果急性胰腺炎组在６，１２ｈ的血清淀粉酶值分别为２７９６±４５３和２４７８±３７８（ＩＵ／Ｌ）；其组织水肿和炎症细胞渗出评分分别为３１４±０６９和３００±０５８．维拉帕米保护组在相应时间的血清淀粉酶值仅为２１１３±４１１和１５７９±３１９（ＩＵ／Ｌ）；其组织水肿和炎症细胞渗出评分分别为１００±０８２和０９０±０３２．两组比较，有显著统计学差异（Ｐ＜００５或００１）．同时，前者电镜下见线粒体肿胀，内质网囊池扩大，自噬泡增加，而后者各细胞器基本正常．结论维拉帕米对实验性急性胰腺炎具有保护作用．
AIM To evaluate the protective effect of verapamil on experimental acute pancreatitis (AP). METHODS Twenty two Wistar rats were randomly assigned into three groups: pseudo operation group ( n =5), AP group ( n =7) and verapamil protection group ( n =10). AP model was induced by injecting 2 0% sodium taurocholate (0 1ml/100g) into the pancreatic bile duct system in rats. Intravenous verapamil (1 0mg/kg.h) was infused for 12 hours prior to induction of AP. The serum amylase levels were tested at the time of 0, 6, and 12 hours after induction of AP. The pancreatic lesions were evaluated by light and electron microscope. RESULTS Serum amylase levels were much lower in verapamil protection group than those in AP group at the time of 6 and 12 hours 2113±411 vs 2796±453, 1579±319 vs 2478±378 (IU/L), P <0 01 . Verapamil treatment definitely improved pancreatic injury including edema (1 00±0 82 vs 3 14±0 69 in scores, P <0 01) and leucocyte infiltration (0 90±0 32 vs 3 00±0 58 in scores, P <0 05). CONCLUSION Verapamil has a protective action on AP.
关键词
胰腺炎
药物疗法
维拉帕米
Pancreatitis/drug therapy\ \ Acute diseases\ \ Verapamil\ \ Disease models, animal
