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环境样品中DNA提取方法的研究进展 被引量：6

Methods for extracting DNA from environmental samples
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摘要 利用微生物降解污染物是废水处理中的常用方法,研究废水处理工艺中微生物的多样性和动态性对于优化废水处理工艺的性能、提高其处理效率具有重要的指导意义。分子生物学方法是研究微生物多样性和动态性的有效方法。由于大多数分子生物学方法都是以提取研究对象的基因组DNA为前提,因此建立一种高效的环境样品DNA的提取方法具有重要作用。综述了从环境样品中提取DNA的主要方法及其研究进展,并提出了一种有效的从活性污泥中提取DNA的具体方法。 The molecular microbial techniques are ideal methods for studying the diversity and dynamics of microbes capable of degrading water pollutants to optimize and/or improve performance of a biological wastewater treatment system. Since the DNA extraction is an essential step of most molecular techniques, an effective method for extracting DNA from environmental samples is very important. The paper presents the fundamentals and status of the four steps （sample pretreatment, cell destruction, DNA extraction and DNA purification） of direct DNA extraction methods and, furthermore, outlines a simple and effective method for extracting DNA from active sludge mixed liquor samples.
作者 陈竹 陈元彩 付时雨 詹怀宇
机构地区 华南理工大学制浆造纸工程国家重点实验室 江南大学工业生物技术教育部重点实验室
出处 《环境污染与防治》 CAS CSCD 北大核心 2007年第7期537-540,544,共5页 Environmental Pollution & Control
基金 国家自然科学基金资助项目(No.20676045) 华南理工大学制浆造纸工程国家重点实验室开放基金资助项目(No.200552) 江南大学工业生物技术教育部重点实验室开放课题(No.KLIB-KF200503)。
关键词 环境样品DNA 直接提取方法 微生物 Environmental samples DNA Direct extraction method Microbes
分类号 X703 [环境科学与工程—环境工程]
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参考文献27

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二级参考文献119

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共引文献223

同被引文献117

引证文献6

二级引证文献35

环境污染与防治
2007年 第7期

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