摘要
目的探讨Hela细胞MTT试验(Hela-MTT)是否可作为家兔眼刺激试验(Draize test)的一种替代方法。方法用该方法对22种化妆品的眼刺激性进行检测,并与1999年卫生部颁布的《化妆品卫生规范》和新颁布的《化妆品卫生规范》(2002版)测定的眼刺激试验最大平均值(MASold和MASnew)分别进行相关性比较。结果Hela-MTT和MASold、MASnew的相关系数分别为-0.811、-0.639。结论Hela-MTT可作为替代方法用于眼刺激性的评价,是比较有潜力的眼刺激替代试验方法。
Objective To delve into feasibility of replacing Draize eye irritation test with Hela-MTT test （MTT being reduced in alive Hela-cells） in evaluations of cosmetics toxicity. Methods 22 kinds of cosmetics were examined in this series by Hela-MTT test and Dralze eye irritation according to procedures and methods of eye irritation test in＇ Hygienic Standards of Cosmetic＇ promulgated by Ministry of Health, PRC in 1999 （ HSC-1999 ） and 2002 （ HSC-2002）. The results obtained in Hela-MTT test were compared with those obtained in Draize eye irritation test. Results The correlation coefficient between Hela-MTT test and Draize eye irritation test in HSC 1999 was -0. 811, and that between Hela-MTT test and Draize eye irritation test in HSC - 2002 was - 0. 639. Conclusion It was suggested that Hela-MTT test could replace Draize eye irritation test in evaluations of cosmetics toxicity.
出处
《实验动物科学》
2007年第2期65-68,共4页
Laboratory Animal Science
关键词
眼刺激试验
替代方法
细胞毒性试验
Eye irritation
Cytotoxicity test
Alternative assay
in vitro
