摘要
采用组织分离法和平板系列稀释法,从健康和感染松枯梢病的松树针叶上分离叶栖细菌891株。通过室内平板对峙培养,初步筛选出16株对枯梢病菌丝有强抑制作用的拮抗细菌,抑菌圈直径均大于20mm。在此基础上选取具有代表性的10个菌株进行温室马尾松盆栽苗防病试验,从中筛选出3株(菌株编号为N29、S(1)102、M(2)21)抑菌圈直径分别为28.5、37.5、31.0mm及防效达60%以上并对松苗无不良影响的拮抗细菌。
Through tissue isolation and plate dilution, 891 bacteria isolations were obtained from healthy and diseased leaves of pines. By the dural culture method on PDA plates with pathogens,16 bacteria isolates had strong antagonism against Sphaeropsis sapinea. Under artificial inoculation 10 representative strains were used on the seedling of pines in green house for identifying their biocontrol effects on S. sapinea. Three（Strain number N29,M（2） 21 and S（1） 102） of 10 isolates had both good roles on PDA plates and seedlings of pine trees. The diameters of their inhabit circle were respectively 28.5,31,37.5 mm and protective effection to the seedlings of pine was over 60%. The result of pathogenicity test demonstrated that they had no harmful influence to the seedling of pine.
出处
《南京林业大学学报（自然科学版）》
CAS
CSCD
北大核心
2007年第4期144-146,共3页
Journal of Nanjing Forestry University：Natural Sciences Edition
基金
国家自然科学基金资助项目(30471392)
高等学校博士学科点专项科研基金资助项目(20040298010)
关键词
松枯梢病
拮抗细菌
分离
筛选
苗期生测
Sphaeropsis sapinea
Antagonistic bacteria
Isolation
Screen
Bioassay
