摘要
目的调查广州市交通干道附近PM2.5的污染状况,并探讨PM2.5有机提取物对BEAS-2B细胞的炎性损伤效应。方法使用PM2.5采样器采集广州市交通干道附近的PM2.5,分析天平称重计算日平均浓度;索氏提取器提取PM2.5吸附的有机物作用于BEAS-2B细胞,ELISA检测IL-8、IL-1β的表达水平。结果广州市PM2.5日平均浓度为0.057～0.194mg/m3,其超标倍数为0.88～2.98;BEAS-2B细胞受到PM2.5有机提取物作用后,随着PM2.5有机提取物染毒剂量的增加,IL-8、IL-1β的分泌也明显增加;且随着各剂量作用时间的延长,IL-8、IL-1β的表达也增高。结论广州市交通干道旁PM2.5污染相当严重;PM2.5有机提取物能够刺激BEAS-2B细胞释放IL-8、IL-1β等炎性因子,吸引大量的炎性细胞释放炎性介质,从而形成气道的炎症损伤。
Objective To analyze the level of particulate matter PM2.5 in Guangzhou, and the effect of the extractable organic matter （EOM） from PM2.5 on BEAS-2B cells. Methods PM2.5 was obtained using a MiniVol Portable Air Sampler. BEAS-2B cells were treated with EOM, and the levels of IL-8 and IL-1β were determined by ELISA. Results The mean daily level of PM2.5 measured near the highway was in the range of 0.057-0.194 mg/m^3. The level was 0.88 to 2.98 folds higher than the standard. In vitro study showed that EOM of PM2.5 increased the expression of IL- 8 and IL-1β in BEAS-2B. Conclusion The level of PM2.5 in the area near highway is high. PM2.5 may stimulate the airway inflammation through the induction of pro-inflammatory eytokines IL-8 and IL-1β.
出处
《热带医学杂志》
CAS
2007年第7期708-710,共3页
Journal of Tropical Medicine
