期刊文献+

原发性肝细胞癌组织中p53和p21^(WAF/CIP1)及MDM2蛋白的表达及其临床意义 被引量:8

Expressions of p53,p21^(WAF/CIP1) and MDM2 pathway proteins in primary hepatocellular carcinoma and their significance
下载PDF
导出
摘要 目的:探讨p53、p21WAF/CIP1及MDM2蛋白在原发性肝细胞癌(hepatocel-lularcarcinoma,HCC)组织中的表达及其临床病理意义。方法:选取115例HCC及其相应的癌旁肝组织构建组织芯片,应用免疫组织化学方法检测p53、p21WAF/CIP1及MDM2蛋白的表达,采用统计学分析它们的表达及其与临床病理参数之间的关系。结果:p53、p21WAF/CIP1及MDM2在HCC组织中的阳性表达率分别为83.5%(96/115)、36.5%(42/115)和48.7%(56/115),同癌旁组织相比,p53、p21WAF/CIP1及MDM2的表达均明显增高,P<0.05。两两相关分析比较,p53表达分别与p21WAF/CIP1和MDM2表达有关;p53、p21WAF/CIP1及MDM2的阳性表达率与患者性别、年龄、肿瘤大小、肿瘤数量、HB-sAg、病理分级、血清AFP浓度和患者生存时间均无关,P均>0.05。结论:p53、p21WAF/CIP1及MDM2在HCC组织中呈增高表达并具有一定的相关性,提示它们可能在HCC的发生发展中发挥重要的作用。 To investigate the expression of p53, p21^WAF/CIP1 and MDM2 proteins in primary hepatocellular carcinoma (HCC) and their significance. METHODS; p53, MDM2 and p21^WAF/CIP1 protein expressions were examined in 115 cases of hepatocellular carcinoma by the tissue array technique and immunohistochemistry. Statistic analysis was performed to figure out their expressions or correlation ammg their expressions and clinicopathological parameters of the patients with HCC. RESULTS: Positive proportions of p53, p21^WAF/CIP1 and MDM2 expressions were 83.5%(96/115), 36.5%(42/115), 48.7%(56/115), respectively. Their expressions in HCC tissues were significantly higher than those in the corresponding liver tissues adjacent to the tumor, P〈0.05. There was a statistic correlation either between p53 and MDM2 expressions in HCC or between p53 and p21^WAF/CIP1 expression in HCC. Statistical significance was not found in correlations among their expressions and the patients' gender, age, tumor size, tumor amount, HbsAg, pathological classification, serum level of alphafetal protein and the patients' survival, P〉0.05. CONCLUSIONS: p53, p21^WAF/CIP1 and MDM2 are increasingly expressed in primary hepatocellular carcinoma, and their expressions are relative. These findings provide an evidence that they may contribute to the pathogenesis of hepatocellular carcinoma.
出处 《中华肿瘤防治杂志》 CAS 2007年第15期1121-1124,共4页 Chinese Journal of Cancer Prevention and Treatment
基金 国家自然科学基金(30471960) 广东省自然科学基金(06021198) 华南地区整合生物科学国家研究中心启动基金
关键词 肝细胞/病理学 肝肿瘤 基因P53 基因p21 蛋白质MDM2 免疫组织化学 carcinoma,hepatocellular/pathology liver neoplasms gene, p53 gene, p21 protein MDM2 lmmunohistochemistry
  • 相关文献

参考文献5

二级参考文献34

  • 1Zhao-You Tang Liver Cancer Institute & Zhongshan Hospital of Fudan University Professor of Surgery Chairman.Liver Cancer Institute of Fudan University(previous Liver Cancer Institute of Shanghai Medical University)136 Yixueyuan Road,Zhongshan Hospital,Shanghai 200032,China..Hepatocellular Carcinoma-Cause,Treatment and Metastasis[J].World Journal of Gastroenterology,2001,7(4):445-454. 被引量:214
  • 2孙保存,赵秀兰,惠京.大肠癌及其癌前病变中细胞凋亡与细胞增殖间关系的原位观察[J].中华医学杂志,1996,76(11):848-851. 被引量:34
  • 3Steck P A, Pershouse M A, Jasser S A, et al. Identification of candidate tumour suppressor gene, MMAC1, at chromosome 10q23. 3 that is mutated in multiple advanced cancer[J]. Nat Genet, 1997,15(8) :356- 362.
  • 4Li J, Yen C, Liaw D, et al. PTEN, a putative protein tyrosine phosphatase gene mutated in human brain,breast, and prostate cancer[J]. Science, 1997, 275 (2) : 1943-1947.
  • 5HuTH, HuangCC,LinPR, etal. Expression and prognostic role of tumor suppressor gene PTEN/MMAC1/TEP1 in hepatocellular carcinoma[J]. Cancer, 2003,97(8) : 1929-1940.
  • 6Tamura M, Gu J, Matsumoto K, et al. Inhibition of cell migration, spreading and focal adhesion by tumor suppressor PTEN[J]. Sciense, 1998,280(9) :1614-1617.
  • 7Di Cristofano A, Pandolfi P P. The multiple roles of PTEN in tumor suppression[J]. Cell, 2000,100 (4): 387 - 390.
  • 8Piao Z, Park C, Park J H,et al. Allelotype analysis of hepatocellular carcinoma[J]. Int J Cancer, 1998,75(1) :29-33.
  • 9Volgelstein B, Lane D, Levine AJ. Surfing the p53 network [J]. Nature, 2000,408(6810): 307-310.
  • 10Inoue S, Tezel E, Nakao A. Molecular diagnosis of pancreatic cancer [ J ]. Hepatogastroenterology, 2001,48 (40): 933-938.

共引文献35

同被引文献79

引证文献8

二级引证文献20

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部