摘要
目的:探讨DNA错配修复基因hMSH_2的表达与子宫内膜癌的关系。方法:选取天津医科大学总医院1981年1月～2001年12月间住院手术子宫内膜癌104例和正常子宫内膜35例(增生期17例,分泌期18例)及8例子宫内膜非典型增生组织标本,采用免疫组化SABC法检测组织中hMSH_2基因蛋白表达情况,并对hMSH_2蛋白表达与子宫内膜癌临床病理特征的关系进行分析。结果:子宫内膜癌、子宫内膜非典型增生、正常子宫内膜增生期和分泌期hMSH_2表达缺失率分别为68.3%、4/8(50.0%)、5.9%和5.6%,子宫内膜非典型增生和子宫内膜癌组织hMSH_2表达缺失率无明显差异(P=0.294),但明显高于正常增生期及分泌期子宫内膜;子宫内膜样腺癌、腺鳞伴鳞状上皮分化、透明细胞癌和浆液性癌hMSH_2表达缺失率分别为73.3%、2/8、2/4和1/2,子宫内膜样腺癌中hMSH_2基因表达缺失率明显高于其它组织学类型(P=0.035)。hMSH_2表达阴性和阳性者5年生存率分别为80.0%、52.0%,10年生存率分别为78.0%、32.0%,hMSH_2基因表达阴性者生存率明显高于阳性表达者。结论:子宫内膜癌扣子宫内膜非典型增生中hMSH_2基因表这缺失率相似,hMSH_2表达缺失可能与子宫内膜癌发生早期有关。hMSH_2基因表达缺失与子宫内膜癌组织学类型中的子宫内膜样腺癌有关,hMSH_2基因表达缺失者预后较好。
Objective： To study the expression of hMSH2 in endomelrial carcinoma. Methods： The expression of hMSH2 protein was analyzed by immunohistochemical technique in 104 cases of endometrial carcinoma, 35 cases of normal endometrium （17 proliferative endometria, 18 secretory endometria） and 8 cases of atypical hyperplasia. The relationship between the expression of hMSH2 and clinicopathologic characteristics of endometrial carcinoma were also investigated. Results： Loss of hMSH2 expression was detected at 5.9% in normal proliferative endometrium, 5.6% in normal secretary endometrium, 50% in atypical hyperplasia and 68.3% in endometrial carcinoma. The differences between normal endometrium and endometrial carcinoma and between normal endometrium and atypical hyperplasia were statistically significant. There was no statistically significant difference between cancer and atypical hyperplasia. The rate of expression of hMSH2 was lower in endometrial carcinoma than that in other histological types （P=0.035）. The 5-year survival rate for patients with negative expression of hMSH2 was 80%, higher than the 52% seen in patients with positive expression. The prognosis lot patients with negative expression of hMSH2 was better. Conclusion： Loss of hMSH2 expression may be related to the early development of endometrial carcinoma. The rate of hMSH2 expression was lowest in endometrial carcinoma. The prognosis of patients with negative expression of hMSH2 was better than that for patients with positive expression.
出处
《中国肿瘤临床》
CAS
CSCD
北大核心
2007年第14期798-802,共5页
Chinese Journal of Clinical Oncology
基金
教育部科学技术研究重点项目(编号:02014)
天津市自然科学基金资助(编号:023610911)
关键词
子宫内膜癌
HMSH2
基因表达
免疫组化
Endometrial careinoma hMSH2 Gene expression Immunohistochemistry
