摘要
目的:探讨海马乙酰胆碱酯酶(AchE)活性与氯胺酮对幼龄大鼠学习记忆功能的影响及其关系。方法:1月龄SD大鼠40只,随机分成2组:氯胺酮组(K组,n=20),对照组(C组,n=20)。K、C组又各分2个亚组,K1、K2组和C1、C2组(每组10只)。K组腹腔注射氯胺酮40mg/kg,随后每隔15min追加1/2首剂量,共追加3次。C组给予生理盐水5mL。K1、C1组于处理后第1周进行水迷宫测试,K2、C2组于处理后第3周进行水迷宫测试,连续7d。末次测试结束后断头处死取海马,行HE染色观察其形态学变化,比色法检测AchE活性。结果:K1组大鼠寻找平台的时间(潜伏期)明显大于C1组(P<0.05或P<0.01),K2组与C2组比较差异无统计学意义(P>0.05)。K1组对平台空间位置的记忆能力低于C1组(P<0.05),K2组与C2组结果相近(P>0.05)。K1组AchE活性明显低于C1组(P<0.01),K2组与C2组比较差异无统计学意义(P>0.05)。光镜下各组大鼠海马神经元结构未见明显异常。结论:腹腔注射氯胺酮可导致幼龄大鼠早期空间学习记忆的损害,但无长期影响,海马区AchE活性的下降可能与其早期影响有关。
To investigate the relationship between the acetylcholinesterase （AchE） activity in hippocampus and the effect of ketamine on spatial learning and memory of the early developing rats. METHODS： Forty one-month old male SD rats were randomly divided into two groups with 20 rats each： ketamine group in which rats received intraperitoneal-ketamine 40 mg/kg and then 20 mg/kg every 15 min 3 times in total; control group （received normal saline 5 mL）. The two groups were further divided into 2 subgroups according to the time when Morris water maze （MWM） testing were started in the 1 st week （C1, K1） or the 3rd week （C2, K2） after the injection. MWM testing was performed in the 1 st week and the 3rd week after the injection. The hippoeampus was dissected away from the brains, then the AchE activity was detected with the eolorimetrie method, and the changes in morphology were observed with HE. RESULTS： （1） For MWM： The latent period of group K1 was signifieantly longer than group C1 （ P 〈 0.05 or P 〈0.01）, but there was no significant difference between group K2 and group C2. In the spatial probe test, the time across the original platform of group K1 was shortel than the group C1 （P 〈0.05）, but there was no significant difference between group K2 and group C2. （2） Fol AchE activity： the AchE activity of group K1 was significandy lower than group C1 （ P 〈 0.05）, but there was nc significant difference between group K2 and group C2. （3） For HE assay： light microscopy showed no abnorma changes of hippocampal neurons in two groups. CON. CLUSION： Ketamine administration impairs spatial le. arning and memory in short time, and a decrease in AchE activity in hippocampus of the rats may have a link with the impairment. However, there is no impairment to le. arning and memory in long time.
出处
《中国临床药理学与治疗学》
CAS
CSCD
2007年第6期672-675,共4页
Chinese Journal of Clinical Pharmacology and Therapeutics
基金
安徽省教育厅资助项目(2003kj320)
关键词
乙酰胆碱酯酶
海马
氯胺酮
学习记忆功能
acetyleholinesterase
hippoeampus
ketamine
learning and memory
