摘要
目的:采用简便易行的方法制备小鼠视网膜新生血管动物模型。方法:新生小鼠20只随机分为两组,实验组于出生后第7-12 d置于75%氧箱内饲养,对照组于正常环境饲养。至第17 d时处死小鼠,摘取眼球,固定切片,行苏木精-伊红(H-E)染色,计数突破视网膜内界膜的内皮细胞核数,免疫组化法观察视网膜各层血管内皮生长因子(VEGF)的表达。结果:实验组平均每个切面突破视网膜内界膜的内皮细胞核数为(25.70±4.90)个,对照组平均为(0.85±1.02)个,两者比较差异有显著性意义(P〈0.01);对照组VEGF染色较实验组减弱。结论:该方法能建立稳定的视网膜新生血管动物模型。
Objective:To establish the mouse model of retinal neovaseularization. Methods : Twenty neonatal C57BL/6J mice were divided into two groups randomly. The treated group was exposed to 75% oxygen for 5 days and then to room air. All animals were sacrificed at postnatal day 17 and both eyes were enucleated. Endothelial cell nuclei extending into the internal limiting membrane were counted via HE stain and the expression of vascular endothelial growth factor (VEGF) was detected via immunohistochemical stain. Results : On average, 25.70 ± 4.90 endothelial cell nuclei were seen in the treated group and 0. 85 ± 1.02 in the control group. The difference was statistically significant( P 〈 0. 01 ). Stain of VEGF was weaker in the control group than in the treated group. Conclusion : The mouse model of retinal neovascularization can be established by this convenient method.
出处
《医学研究生学报》
CAS
2007年第3期257-258,I0004,共3页
Journal of Medical Postgraduates
基金
江苏省六大人才高峰重点课题基金资助项目(批准号:2005A3)