摘要
以啤酒废酵母为原料,采用自溶结合外加酶法制备酵母抽提物。外加蛋白酶可以提高产物中固形物及氨基氮得率;添加5mg/g干酵母的Protamex时,固形物得率和氨基氮得率分别比不加酶对照提高了24.7%和82.8%;添加5mg/g干酵母的Neurase时,氨基氮得率提高了62.3%。同时添加0.5mg/g干酵母的溶壁酶Lywallzyme和5mg/g干酵母的木瓜蛋白酶处理,上清液氨基氮含量达4580mg/L;固形物得率达60%。
Yeast extract was produced from spent brewer＇s yeast of a beer factory by enzymatic treatments using proteases and lysing-wall enzymes. Adding 0.5 % （w/w） Protamex, the solid recovery and amino nitrogen recovery increased by 24.7 % and 82.8 % respectively. When the cells were treated using the combination of 0.05 %
（w/w） Lywallzyme and 0.5 % （w/w） papain, the solid recovery reached 60 % and the amino nitrogen content of the autolysates reached 4580 mg/L.
出处
《食品研究与开发》
CAS
北大核心
2007年第8期8-11,共4页
Food Research and Development
基金
广州市科技计划项目(2006Z3-E0271)
关键词
啤酒废酵母
酵母抽提物
酶促自溶
固形物得率
氨基氮
Spent brewer＇s yeast
yeast extract
enzymatic hydrolysis
solid recovery
amino nitrogen
