摘要
根据GenBank中已发表的传染性支气管炎病毒（IBV）S1基因,设计并合成了1对引物,采用RT-PCR技术扩增鸡传染性支气管炎病毒河南分离株（HN0501）S1基因,并进行克隆和测序.结果表明,所克隆的片段大小为1739 bp,包含S1基因和部分S2基因,S蛋白切割识别位点序列为RRSRR.序列比较分析发现,IBV HN0501株S1基因与澳大利亚N1株、吉林JAAS株S1基因核苷酸同源性分别为99.6%,99.8%,氨基酸同源性均为99.3%,而与其它支气管炎病毒株核苷酸同源性为66.4%-85.2%.通过IBV S1基因系统进化分析,结果发现HN0501株与N1株、JAAS株的亲缘关系近,而与其它支气管炎病毒株的亲缘关系均较远.进一步证实IBVHN0501株为IBV肾型株.
One pair of primers was designed and synthesized according to chicken infectious bronchitis virus S1 gene sequences in the GenBank. S1 gene of the infectious bronchitis virus （IBV） Henan isolate（ HN0501 ） was amplified by using reverse transcription-polymerase chain reaction（RT-PCR）. The purified PCR product was cloned into pGEM-T Easy vector and then was sequenced. The results show that S1 gene sequence of HN0501 strain consisted of 1739 bp. Sequence comparison and analysis Show S1 genes of HN0501 and other strains of IBV published previously in the GenBank, shared 99.6% , 99.8% homology with N1, JAAS, respectively, and 66.4% -85.2% homology with that of other strains of IBV. Evolution analysis indicated that the HN0501 isolate had near relation to N1 and JAAS and far relation to other strains of IBV. The results showed that the HN0501 strain is a nephropathogenic IBV strain.
出处
《河南农业大学学报》
CAS
CSCD
北大核心
2007年第3期295-299,共5页
Journal of Henan Agricultural University
基金
国家"十五"食品安全重大攻关项目(2001BA804A30-11)
