摘要
用萌蘖芽为外植体对黄藤进行组培快繁试验,结果表明,高浓度的6BA及多激素组合有利于丛芽诱导,但在继代增殖过程中需要适当降低6BA的浓度及除去2,4-D以利于芽伸长壮苗;0.25～0.50MS诱导根效果好;培养基中加入0.1 g/L活性炭显著提高生根率;NAA诱导的根过粗,不利于幼苗生长和移植成活;而IBA诱导的根细长,形态上接近自然根,幼苗易长高,移栽易成活。
Experiment on vitro propagation of Daemonorops margaritae by sucker as explant showed that high concentration of 6BA and multiple hormones combination could promote cluster induction. But lowering concentration of 6BA and removing 2,4-D was necessary for shoot elongation of subculture proliferation. The result showed that the 0.25MS-0.5MS had the best effect of root induction. Rooting rate could also be improved effectively with treatment of 0.1g/L activated carbon. Root induced by NAA was too thick and had low survival rate of transplanting. But that induced by IBA were thin and natural and had higher survival rate.
出处
《浙江林业科技》
北大核心
2007年第3期15-18,共4页
Journal of Zhejiang Forestry Science and Technology
基金
"十一五"国家科技支撑项目"棕榈藤无性快繁工艺研究"及国家高技术研究发展计划(863计划)项目(2006AA100109)
关键词
黄藤
萌蘖芽
外植体
组培快繁
Daemonorops margaritae
sucker
explant
tissue culture
