摘要
目的 建立同时测定丹参药材及其制剂中丹参素、原儿茶醛、丹酚酸A、丹酚酸B4种酚酸类成分含量的RP-HPLC色谱法。方法色谱柱：InertsilC8-3柱;流动相：乙腈-水-甲酸（24∶76∶0.4）;流速：1.0mL·min^-1;检测波长：285nm。结果在此色谱条件下4种酚酸类成分可完全分离。丹参素、原儿茶醛、丹酚酸A、丹酚酸B的线性范围分别为：8-400（r=0.9998）,4-200（r=0.9994）,4-200（r=1.0000）和4-200mg·L^-1（r=1.0000）。4种成分在药材和制剂中的回收率均在98.5%-101.0%,RSD（2.0%。结论该方法简便、准确、快速,可同时测定丹参药材及其制剂中4种酚酸类成分的含量。
OBJECTIVE To develop a HPLC method for the simultaneous determination of danshensu, protocatechuic aldehyde, salvianolic acid A, salvianolic acid B in Salvia miltiorrhiza Bge. and its preparations. METHODS The analysis was carried on a column of Inertsil Cs-3 with a mobile phase of acetonitrile-water-formic acid（ 24： 76： 0. 4） at the flow rate of 1.0 mL· min^-1. The detection wavelength was 285 nm. RESULTS The linearity was obtained over the range of 8-400 mg·L^-1 （ r = 0. 999 8 ） for danshensu, 4-200 mg^-L 1 （ r = 0. 999 4） for protocatechuic aldehyde, 4-200 mg·L^- 1 （ r = 1. 000 0） for salvianolic acid A and 4-200 mg·L^- 1 （ r = 1. 000 0） for salvianolic acid B, respectively. The average recoveries of four phenolic acids in Salvia miltiorrhiza Bge. And its preparations determined were all in 98.5% - 101.0%, and the RSDs were less than 2. 0%. CONCLUSION The method is simple, accurate and rapid. It may be suitable for the usual quality control method of Salvia miltiorrhiza Bge. and its preparations.
出处
《中国药学杂志》
CAS
CSCD
北大核心
2007年第14期1108-1111,共4页
Chinese Pharmaceutical Journal
关键词
高效液相色谱法
丹参
丹参素
原儿茶醛
丹酚酸A
丹酚酸B
含量测定
HPLC
Salvia miltiorrhiza Bge.
danshensu
protocatechuic aldehyde
salvianolic acid A
salvianolic acid B
quantitative analysis
