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活化玻碳电极直接测定注射液多巴胺的含量 被引量:2

Determination of dopamine content in injection with the activated glassy carbon electrode
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摘要 目的:研究多巴胺(DA)在活化玻碳电极(AGCE)上的电化学行为,建立一种测定多巴胺(DA)的电化学分析方法。方法:玻碳电极在0.1 mol·L^(-1)磷酸盐缓冲液(pH 7.0)中活化,用循环伏安法研究 DA 在活化玻碳电极上的氧化还原特性,用微分脉冲伏安法直接测定 DA 的含量。结果:DA 在活化玻碳电极上的循环伏安图具有一对灵敏的氧化还原峰,峰电位分别为0.167 V 和0.217 V(vs.SCE)。与裸玻碳电极相比,该电极对 DA 的氧化具有良好的电催化作用。微分脉冲伏安法测定 DA 的氧化峰电流与其浓度在2个区间成正比,分别为1.0×10^(-6)~2.5×10^(-5)~mol·L^(-1)和2.5×10^(-5)~2.5×10^(-4)mol·L^(-1),线性相关系数分别为0.9931和0.9938,检出限5.0×10^(-7)~mol·L^(-1)。抗坏血酸(AA)和尿酸(UA)对 DA 的测定均没有干扰。结论:该方法操作简单方便,重现性较好,用于测定多巴胺注射液中 DA 的含量,结果令人满意。 Objective : To investigate the electrochemical behaviors of dopamine (DA) at the activated glassy carbon electrode (AGCE) and establish a electroanalytical technique for the determination of DA. Methods:The glassy carbon electrode was activated electrochemically in 0. 1 mol·L^-1 phosphate buffer solution ( pH 7. 0). The redox characteristics of DA were investigated at the AGCE by cyclic vohammetry (CV). The dopamine content was determined by differential pulse vohammetry (DPV). Results:A pair of sensitive redox peaks were observed at 0. 167 V and 0. 217 V(vs. SCE),with the anodic and cathodic peak respectively,in 0. 1 mol·L^-1 phosphate buffer solution (pH 7. 0). Compared with the bare glassy carbon electrode (GCE), the AGCE showed an excellent electrocatalytic activity for the oxidation of DA. The anodic peak current was proportional to the concentration of DA in two sections,over the range of 1.0 × 10^-6 to 2. 5 × 10^-5 mol·L^-1 and 2. 5 × 10^-5 to 2. 5 × 10^-4 mol·L^-1 respectively,and with the respective correlation coefficients of 0. 9931 and 0. 9938. The detection limit was 5.0 × 10^ -7 mol·L^-1. The AGCE is easy to prepare with good reproducibility. Ascorbic acid (AA) and uric acid (UA) have no interference to the determination of DA. Conclusion:The proposed method is simple, sensitive and reproducible and was applied to measure the content of DA in injection with a good recovery.
出处 《药物分析杂志》 CAS CSCD 北大核心 2007年第7期968-972,共5页 Chinese Journal of Pharmaceutical Analysis
基金 国家自然科学基金(29975025)
关键词 活化玻碳电极 多巴胺 注射液 循环伏安法 微分脉冲伏安法 activated glassy carbon electrode dopamine injection cyclic voharmnetry differential pulse voltammetry
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