摘要
目的:建立 HPLC 法测定金甲王颗粒中柚皮苷含量。方法:采用 Dikma Diamonsil^(TM)C_(18)色谱柱(4.6mm×250mm,5μm),以0.05moL·L^(-1)磷酸二氢钾-乙腈-浓氨试液(80:20:0.2)为流动相,流速1mL·min^(-1),检测波长为283nm。结果:柚皮苷进样量在0.28～1.41μg范围内与峰面积具有良好的线性关系(r=0.9999);平均回收率为99.4%,RSD=1.4%(n=6)。结论:本方法操作简便,结果准确,可用于该品种的质量控制。
Objective：To develop an HPLC method for determination of naringin in Jinjiawang granules. Method： Dikma DiamonsilTM Cls column was adopted with a mixture of 0. 05 moL·L^-1 potassium dihydrogen phosphate- acetonitrile- strong ammonia （80： 20： 0. 2） as the mobile phase,the flow rate was 1 mL · min^-1 ,and the detection wavelength was 283 nm. Results：The calibration range was 0. 28 - 1.41 μg for naringin （r = 0. 9999 ）. The average recovery was 99.4% ,RSD = 1.4% （n = 6 ）. Conclusion： The method is rapid ,accurate and reliable ,and can be used for the quality control of Jinjiawang granules.
出处
《药物分析杂志》
CAS
CSCD
北大核心
2007年第7期1078-1080,共3页
Chinese Journal of Pharmaceutical Analysis
