摘要
目的 研究呼吸道合胞病毒（RSV）感染后大鼠脊髓背根节感觉神经元结构蛋白的表达变化，阐明气道神经可塑性改变的分子机制。方法 1-2周龄SD大鼠共30只，区组随机分为空白对照组、RSV感染组和神经生长因子（NGF）抗体干预组，每组10只。RSV感染组和NGF抗体干预组采用每周1次RSV滴鼻法制作RSV感染模型，NGF抗体干预组先腹膜腔内注射山羊抗大鼠-βNGF抗体，3 h后按照RSV感染组相同的方法进行RSV感染。在第8周进行气道反应性测定，并留取C7-T5脊髓背根节组织，采用免疫组化和RT-PCR检测C7-T5脊髓背根节突触囊泡素的表达。结果 RSV感染组对不同浓度组胺（0.01-0.16 mg/mL）的气道反应性均显著高于空白对照组（P均〈0.05），NGF抗体干预组在≥0.08 mg/mL组胺刺激后气道反应性显著低于RSV感染组，在≥0.04 mg/mL组胺刺激后显著高于正常对照组（P均〈0.05）。RSV感染后突触囊泡素表达较对照组显著升高；NGF抗体干预后突触囊泡素的表达虽低于RSV感染组，但仍高于正常对照组。突触囊泡素mRNA表达亦呈现类似改变。相关分析显示C7-T5脊髓背根节突触囊泡素的蛋白表达与气道反应性呈正相关关系（r=0.813，P〈0.05）。结论 反复RSV感染后脊髓背根节感觉神经元突触囊泡素的表达增高，并与气道反应性增高密切相关。
Objective To investigate the expression of synaptophysin in the C7-T5 spinal adorsal root ganglion cells of SD rats after recurrent respiratory syncytial virus（RSV） infection and attempt to elucidate the molecular mechanism of airway neuronal plasticity. Methods Thirty SD rats were randomly divided into 3 groups with 10 rats in each group： a control group, a RSV-infected group（ the rats were infected with 5 × 10^4 TCID50/0.1 mL RSV once a week）,and a NGF-antibody prevented group [the rats were infected with RSV 3 hours after a peritoneal injection of anti-rat beta-nerve growth factor （β-NGF） antibody ]. The airway responsiveness was measured after 8 weeks. Then the C7-T5 spinal adorsal root ganglia were gained for synaptophysin expression measurement by immunohistochemistry and RT-PCR.Results The airway responsiveness in the RSV-infected group was higher than which in the control group at all levels of histamine concentration （0.01-0.16 mg/mL）, and which in the NGF-anfibody prevented group was lower than the RSV-infected group at dose of histamine ≥0.08 mg/mL and higher than the control group at dose of histamine ≥0.04 mg/mL（all P 〈 0.05）. The protein and mRNA expression of synaptophysin in the C7-T5 spinal adorsal root ganglia was increased by recurrently RSV-infection and was inhibited by NGF antibedy partly（ P 〈 0.05）. The synaptophysin expression showed a positive correlation to airway responsiveness （ r = 0.813, P 〈 0.05）. Conclusions The expression of synaptophysin in the C7-T5 spinal adorsal root ganglion cells of SD rats is increased after recurrent RSV infection which may be involved in the pathogenesis of airway hyperresponsiveness.
出处
《中国呼吸与危重监护杂志》
CAS
2007年第4期294-297,302,I0002,共6页
Chinese Journal of Respiratory and Critical Care Medicine
基金
国家自然科学基金资助(编号:30470756)
关键词
呼吸道合胞病毒
神经可塑性
气道高反应性
脊髓背根节
突触囊泡素
Respiratory syncyfial viruses
Neuronal plasticity
Airway hyperresponsiveness
Adorsal root ganglia
Synaptophysin
