摘要
对２株Ｂ群脑膜炎奈瑟氏球菌的铁调蛋白的表达及其性质作了研究。在流脑半综合培养基中加人铁螯合剂，诱导了铁调蛋白的表达，并比较了三种铁螯合剂的效果。结果表明，ＥＤＤＨＡ是最令人满意的螯合剂。ＥＬＩＳＡ实验证实含有铁调蛋白的外膜蛋白可以增强对小白鼠的免疫原性。Ｗｅｓｔｅｒｎ－ｂｌｏｔ实验证明有两种铁调蛋白是有效的抗原成分，分子量分别在７５ｋＤａ和８５ｋＤａ附近。由分子量来推算，分别应为ＦｅＲＰ－７０及ＴＢＰ２。
Our research here was focused on the expression and characteristics of the iron regulated proteins (IRPs) of two Neisseria meningitidis strains,which belong to group B.We induced the expression of IRPs with the addition of iron chelators in our semi synthetic media(SSM)and compared the effects of three kinds of chelators.EDDHA was shown to be most satisfactory.ELISA confirmed the imunogenicity of IRPs.And Western blot assay proved that two kinds of IRPs made this cotribution.Deduced from their molecular weights,one might be TBP2 and the other FeRP-70.
出处
《微生物学免疫学进展》
1997年第1期16-19,共4页
Progress In Microbiology and Immunology
关键词
脑膜炎
奈瑟氏球菌
铁调蛋白
菌苗
研制
Neisseria meningitidis Iron regulated proteins Transferrin binding protein 1 and 2 Vaccine
