摘要
目的观察环境雌激素辛基酚(OP)和三羟异黄酮(GEN)对乳腺癌细胞株(MDA-MB-231)增殖和DNA损伤的影响。方法以2,8和32μmol/LOP或GEN与乳腺癌细胞共培养24,48或72h,用四甲基偶氮噻唑蓝(MTT)试验和单细胞凝胶电泳观察环境雌激素影响细胞增殖和DNA损伤程度。结果OP能明显抑制细胞增殖,DNA损伤明显增加,并呈剂量-效应和时间-效应关系。2,8μmol/LGEN促进细胞的增殖,DNA损伤无明显改变,32μmol/L却明显抑制细胞的增殖,彗星拖尾长度明显增长,但增长效应不及OP。结论OP和高浓度GEN通过损伤MDA-MB-231细胞DNA导致细胞增殖受到抑制,但GEN损伤效应不及OP。
Objective To observe the effect of environmental estrogenic chemicals（EECs） octylphenol and genistein on DNA damage and proliferation of MDA-MB-231 breast cancer cells. Methods MDA-MB-231 breast cancer cell were cultured with 2, 8 and 32 μ mol/L octylphenol or genistein on 24, 48 or 72 h. The proliferation and DNA damage of MDA-MB-231 breast cancer cell were observed by MTT test and single cell gel electrophoresis assay. Results Octylphenol could inhibit the proliferation of MDA-MB-231 cell and increase the DNA damage of breast cancer cells significantly and show daseresponse and time-response. 2 and 8 μ mol/L genistein could promote the proliferation of MDA-MB-231 cell, but the DNA damage did not change significantly. 32 μ mol/L genistein could inhibit the proliferation of MDA-MB-231 cell and increase comet tail length of breast cancer significantly. The comet tail length （CTL） in genisteintreated group was not as long as that in octylphenlo-treated group. Conclusion Octylphenol and high - concentration of genistein can induce DNA damage and inhibit the proliferation of MDA-MB-231 breast cancer cells, but DNA damage in genistein - treated group is not as serious as that in octylphenoltreated group.
出处
《中国公共卫生》
CAS
CSCD
北大核心
2007年第8期935-937,共3页
Chinese Journal of Public Health
关键词
DNA损伤
乳腺癌细胞
环境雌激素
DNA damage
breast cancer cell
environmental estrogenic chemicals
